Description
Recombinant Human POMGNT1 Protein (His Tag) | PKSH032917 | Gentaur US, UK & Europe Disrtribition
Synonyms: Protein O-Linked-Mannose Beta-1;2-N-Acetylglucosaminyltransferase 1; POMGnT1; UDP-GlcNAc:Alpha-D-Mannoside Beta-1;2-N-Acetylglucosaminyltransferase I.2;POMGNT1; MGAT1.2
Active Protein: N/A
Activity: Recombinant Human Protein O-Linked-Mannose beta-1 2-N-Acetylglucosaminyltransferase 1 is produced by our Mammalian expression system and the target gene encoding Leu59-Thr660 is expressed with a 6His tag at the C-terminus.
Protein Construction: Recombinant Human Protein O-Linked-Mannose beta-1 2-N-Acetylglucosaminyltransferase 1 is produced by our Mammalian expression system and the target gene encoding Leu59-Thr660 is expressed with a 6His tag at the C-terminus.
Fusion Tag: C-6His
Species: Human
Expressed Host: Human Cells
Shipping: This product is provided as liquid. It is shipped at frozen temperature with blue ice/gel packs. Upon receipt, store it immediately at<-20°C.
Purity: > 90 % as determined by reducing SDS-PAGE.
Endotoxin: < 1.0 EU per µg as determined by the LAL method.
Stability and Storage: Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles.
Molecular Mass: 69.3 kDa
Formulation: Supplied as a 0.2 μm filtered solution of 20mM TrisHCl, 150mM NaCl, 10% Glycerol, pH 8.5.
Reconstitution: Not Applicable
Background: Protein O-Linked-Mannose β-1 2-N-Acetylglucosaminyltransferase 1 (POMGNT1) belongs to the Glycosyltransferase 13 family. Amino acid residues between 299-311 are important for both protein expression and enzymatic activity. The minimal catalytic domain is located between positions 299-651. It is suggested that the stem domain of the soluble form is unnecessary for activity, but that some amino acids play a crucial role in the membrane-bound form. Defects in POMGNT1 are the cause of muscular dystrophy-dystroglycanopathy congenital with brain and eye anomalies type A3 (MDDGA3).
Research Area: Tags & Cell Markers
