Recombinant Human PKC nu/PRKD3 Protein (GST Tag) | PKSH030402

Recombinant Human PKC nu/PRKD3 Protein (GST Tag) | PKSH030402 | Gentaur US, UK & Europe Disrtribition

Synonyms: EPK2;nPKC-NU;PKC-NU;PKD3;PRKCN

Active Protein: N/A

Activity: A DNA sequence encoding the full length of human PRKD3 (NP_005804.1) (Met 1-Pro 890) was expressed with the GST tag at the N-terminus.

Protein Construction: A DNA sequence encoding the full length of human PRKD3 (NP_005804.1) (Met 1-Pro 890) was expressed with the GST tag at the N-terminus.

Fusion Tag: N-GST

Species: Human

Expressed Host: Baculovirus-Insect Cells

Shipping: This product is provided as liquid. It is shipped at frozen temperature with blue ice/gel packs. Upon receipt, store it immediately at<-20°C.

Purity: > 85 % as determined by reducing SDS-PAGE.

Endotoxin: < 1.0 EU per µg as determined by the LAL method.

Stability and Storage: Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles.

Molecular Mass: 126.7 kDa

Formulation: Supplied as sterile 20mM Tris, 500mM NaCl, 10mM Reduced Glutathione, pH 7.4

Reconstitution: Not Applicable

Background: Serine/threonine-protein kinase D3, also known as Protein kinase C nu type, Protein kinase EPK2, PRKD3, EPK2 and PRKCN, is a cytoplasm and membrane protein which belongs to the protein kinase superfamily, CAMK Ser/Thr protein kinase family and PKD subfamily. PRKD3 / PRKCN contains one PH domain, two phorbol-ester/DAG-type zinc fingers and one protein kinase domain. Protein kinase C (PKC) is a family of serine- and threonine-specific protein kinases that can be activated by calcium and the second messenger diacylglycerol. PKC family members phosphorylate a wide variety of protein targets and are known to be involved in diverse cellular signaling pathways. They also serve as major receptors for phorbol esters, a class of tumor promoters. Each member of the PKC family has a specific expression profile and is believed to play a distinct role. PRKD3 / PRKCN converts transient diacylglycerol (DAG) signals into prolonged physiological effects, downstream of PKC. It is involved in resistance to oxidative stress. PRKD3 / PRKCN is activated by DAG and phorbol esters. Phorbol-ester/DAG-type domains 1 and 2 bind both DAG and phorbol ester with high affinity and mediate translocation to the cell membrane. Autophosphorylation of Ser-735 and phosphorylation of Ser-731 by PKC relieves auto-inhibition by the PH domain. PRKD3 / PRKCN can be activated rapidly by the agonists of G protein-coupled receptors. It resides in both cytoplasm and nucleus, and its nuclear accumulation is found to be dramatically enhanced in response to its activation. PRKD3 / PRKCN can also be activated after B-cell antigen receptor (BCR) engagement, which requires intact phospholipase C gamma and the involvement of other PKC family members.

Research Area: Signal Transduction,