Description
Recombinant Human PFKFB3 (N-6His) | PKSH033971 | Gentaur US, UK & Europe Disrtribition
Synonyms: IPFK2; PFK2; iPFK-2; PFK/FBPase 3
Active Protein: N/A
Activity: Recombinant Human 6-phosphofructo-2-kinase/fructose-2, 6-bisphosphatase 3 is produced by our E.coli expression system and the target gene encoding Met1-His520 is expressed with a 6His tag at the N-terminus.
Protein Construction: Recombinant Human 6-phosphofructo-2-kinase/fructose-2, 6-bisphosphatase 3 is produced by our E.coli expression system and the target gene encoding Met1-His520 is expressed with a 6His tag at the N-terminus.
Fusion Tag: N-6His
Species: Human
Expressed Host: E.coli
Shipping: This product is provided as liquid. It is shipped at frozen temperature with blue ice/gel packs. Upon receipt, store it immediately at<-20°C.
Purity: > 95 % as determined by reducing SDS-PAGE.
Endotoxin: < 1.0 EU per µg as determined by the LAL method.
Stability and Storage: Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles.
Molecular Mass: 61.8 kDa
Formulation: Supplied as a 0.2 μm filtered solution of 50mM Tris-HCl, 50mM KCl, 1mM DTT, 0.1mM EDTA, 20% Glycerol, pH 8.0.
Reconstitution: Not Applicable
Background: Fructose-2, 6-biphosphatase 3, also known as 6-phosphofructo-2-kinase or PFK2 or PFKFB3, involved in both the synthesis and degradation of fructose-2, 6-bisphosphate, a regulatory molecule that controls the activity of the enzymes phosphofructokinase 1 (PFK-1) and fructose 1, 6-bisphosphatase (FBPase-1) to regulate glycolysis and gluconeogenesis. Highly phosphorylated PFKFB3 protein was found in human tumor cells, vascular endothelial cells, and smooth muscle cells. Because of its proto-oncogenic character, the PFK-2/FBPase-2 of the PFKFB3 gene is assumed to play a critical role in tumorigenesis. PFKFB3 also plays a crucial role in the progression of cancerous cells by enabling their glycolytic pathways even under severe hypoxic conditions, which makes it a potential target for cancer therapy.
Research Area: Signal Transduction, Cardiovascular, Neuroscience, Cancer, metabolism,
