Description
Recombinant Human PAPS Synthase 1/PAPSS1 Protein (His Tag) | PKSH032852 | Gentaur US, UK & Europe Disrtribition
Synonyms: Bifunctional 3'-phosphoadenosine 5'-phosphosulfate synthase 1;PAPS synthase 1;PAPSS 1;Sulfurylase kinase 1;SK 1;ATPSK1; PAPSS
Active Protein: N/A
Activity: Recombinant Human Bifunctional 3'-phosphoadenosine 5'-phosphosulfate synthase 1 is produced by our Mammalian expression system and the target gene encoding Met1-Ala624(O43252) is expressed with a 6His tag at the C-terminus.
Protein Construction: Recombinant Human Bifunctional 3'-phosphoadenosine 5'-phosphosulfate synthase 1 is produced by our Mammalian expression system and the target gene encoding Met1-Ala624(O43252) is expressed with a 6His tag at the C-terminus.
Fusion Tag: C-6His
Species: Human
Expressed Host: Human Cells
Shipping: This product is provided as liquid. It is shipped at frozen temperature with blue ice/gel packs. Upon receipt, store it immediately at<-20°C.
Purity: > 95 % as determined by reducing SDS-PAGE.
Endotoxin: < 1.0 EU per µg as determined by the LAL method.
Stability and Storage: Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles.
Molecular Mass: 71.9 kDa
Formulation: Supplied as a 0.2 μm filtered solution of 20mM Tris, 100mM NaCl, 20% glycerol, pH 8.0.
Reconstitution: Not Applicable
Background: PAPSS1 is a bifunctional enzyme with both ATP sulfurylase and APS kinase activity. In the N-terminal section; it belongs to the APS kinase family; while the C-terminal section belongs to the sulfate adenylyltransferase family. PAPSS1 can be inhibited by chlorate; and is expressed in many tissues; such as high endothelial venules (HEV) cells and in cartilage. PAPSS1 mediates two steps in the sulfate activation pathway. The first step is the transfer of a sulfate group to ATP to yield adenosine 5'-phosphosulfate (APS); and the second step is the transfer of a phosphate group from ATP to APS yielding 3'-phosphoadenylylsulfate. In mammals; PAPS is the sole source of sulfate; APS appears to be only an intermediate in the sulfate-activation pathway. PAPSS1 also involved in the biosynthesis of sulfated L-selectin ligands in endothelial cells.
Research Area: Cell biology
