Recombinant Human NSE/ENO2 Protein (His Tag) | PKSH033672

Recombinant Human NSE/ENO2 Protein (His Tag) | PKSH033672 | Gentaur US, UK & Europe Disrtribition

Synonyms: Gamma-enolase;2-phospho-D-glycerate hydro-lyase;Enolase 2;Neural enolase;Neuron-specific enolase;NSE;ENO2

Active Protein: N/A

Activity: Recombinant Human NSE is produced by our E.coli expression system and the target gene encoding Met1-Leu434 is expressed with a 6His tag at the N-terminus.

Protein Construction: Recombinant Human NSE is produced by our E.coli expression system and the target gene encoding Met1-Leu434 is expressed with a 6His tag at the N-terminus.

Fusion Tag: N-His

Species: Human

Expressed Host: E.coli

Shipping: This product is provided as lyophilized powder which is shipped with ice packs.

Purity: > 95 % as determined by reducing SDS-PAGE.

Endotoxin: < 1.0 EU per μg as determined by the LAL method.

Stability and Storage: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.

Molecular Mass: 49.4 kDa

Formulation: Lyophilized from a 0.2 μm filtered solution of 20mM Tris, 100mM KCl, 5mM MgSO4, pH 7.5.

Reconstitution: Please refer to the printed manual for detailed information.

Background: Gamma-enolase; also known as Enolase 2; belongs to the enolase family. The alpha/alpha homodimer of ENO2 is expressed in embryo and in most adult tissues. The alpha/beta heterodimer and the beta/beta homodimer are found in striated muscle; and the alpha/gamma heterodimer and the gamma/gamma homodimer in neurons. During ontogenesis; there is a transition from the alpha/alpha homodimer to the alpha/beta heterodimer in striated muscle cells; and to the alpha/gamma heterodimer in nerve cells. Levels of ENO2 increase dramatically in cardiovascular accidents; cerebral trauma; brain tumors and Creutzfeldt-Jakob disease. ENO2 has neurotrophic and neuroprotective properties on a broad spectrum of central nervous system (CNS) neurons. It binds to cultured neocortical neurons and promotes cell survival in a calcium-dependent manner.

Research Area: Signal Transduction, Tags & Cell Markers, Neuroscience, Cancer, Developmental Biology, epigenetics and nuclear signal, metabolism, Stem cells