Recombinant Human NOL3 Protein | PKSH032827

Recombinant Human NOL3 Protein | PKSH032827 | Gentaur US, UK & Europe Disrtribition

Synonyms: Nucleolar Protein 3; Apoptosis Repressor With CARD; Muscle-Enriched Cytoplasmic Protein; Myp; Nucleolar Protein of 30 kDa; Nop30

Active Protein: N/A

Activity: Recombinant Human Nucleolar Protein 3 is produced by our E.coli expression system and the target gene encoding Met1-Ser208 is expressed.

Protein Construction: Recombinant Human Nucleolar Protein 3 is produced by our E.coli expression system and the target gene encoding Met1-Ser208 is expressed.

Fusion Tag: N/A

Species: Human

Expressed Host: E.coli

Shipping: This product is provided as liquid. It is shipped at frozen temperature with blue ice/gel packs. Upon receipt, store it immediately at<-20°C.

Purity: > 90 % as determined by reducing SDS-PAGE.

Endotoxin: < 1.0 EU per µg as determined by the LAL method.

Stability and Storage: Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles.

Molecular Mass: 22.6 kDa

Formulation: Supplied as a 0.2 μm filtered solution of 25mM TrisHCl, 1mM DTT, 1mM EDTA, 2mM β-ME, 20% Glycerol, pH 7.5.

Reconstitution: Not Applicable

Background: Nucleolar protein 3 is encoded by NOL3 gene. Multiple transcript variants encoding different isoforms have been found for this gene. So far; Nucleolar protein 3 has show to have two Isoforms. Isoform 1 may be involved in RNA splicing. Isoform 2 functions as an apoptosis repressor that blocks multiple modes of cell death. It inhibits extrinsic apoptotic pathways through two different ways. Firstly; it by interacting with FAS and FADD upon FAS activation blocking death-inducing signaling complex (DISC) assembly. Secondly by interacting with CASP8 in a mitochondria localization- and phosphorylation-dependent manner; limiting the amount of soluble CASP8 available for DISC-mediated activation. It has been shown to down-regulate the enzyme activities of caspase 2; caspase 8 and tumor protein p53.

Research Area: Cell biology