Recombinant Human Neuroligin 1/NLGN1 Protein (His Tag) | PKSH031062

Recombinant Human Neuroligin 1/NLGN1 Protein (His Tag) | PKSH031062 | Gentaur US, UK & Europe Disrtribition

Synonyms: Neuroligin-1; NLGN1; KIAA1070;NL1

Active Protein: N/A

Activity: A DNA sequence encoding the human NLGN1 (NP_055747.1) extracellular domain (Met 1-Ser 677) was expressed; fused with a polyhistidine tag at the C-terminus.

Protein Construction: A DNA sequence encoding the human NLGN1 (NP_055747.1) extracellular domain (Met 1-Ser 677) was expressed; fused with a polyhistidine tag at the C-terminus.

Fusion Tag: C-His

Species: Human

Expressed Host: HEK293 Cells

Shipping: This product is provided as lyophilized powder which is shipped with ice packs.

Purity: > 97 % as determined by reducing SDS-PAGE.

Endotoxin: < 1.0 EU per µg as determined by the LAL method.

Stability and Storage: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.

Molecular Mass: 72 kDa

Formulation: Lyophilized from sterile PBS, pH 7.4

Reconstitution: Please refer to the printed manual for detailed information.

Background: Neuroligin 1 (NLGN1) belongs to the type-B carboxylesterase/lipase family; is a synaptic cell-adhesion molecule that is enriched in postsynaptic densities where it may recruit receptors; channels; and signal-transduction molecules to synaptic sites of cell adhesion. Neuroligins consist of five members (NLGN1; NLGN2; NLGN3; NLGN4 and NLGN4Y); which interact with beta-neurexins and this interaction is involved in the formation of functional synapses. The extracellular domain of functional Neuroligin 1 associates as a dimer when analyzed by sedimentation equilibrium. Neuroligin 1 has a unique N-linked glycosylation pattern in the neuroligin family; and glycosylation and its processing modify neuroligin activity. Neuroligin 1 is a potent trigger for the de novo formation of synaptic connections; and it has recently been suggested that it is required for the maturation of functionally competent excitatory synapses. The persistent expression of Neuroligin 1 is required for the maintenance of NMDAR-mediated synaptic transmission; which enables normal development of synaptic plasticity and long-term memory in the amygdala of adult animals.

Research Area: Neuroscience