Description
Recombinant Human Neuritin/NRN1 Protein (Baculovirus, His Tag) | PKSH030790 | Gentaur US, UK & Europe Disrtribition
Synonyms: dJ380B8.2;MGC44811;NRN
Active Protein: N/A
Activity: A DNA sequence encoding the mature form of human NRN1 (Q9NPD7) (Met 1-Asn 115) was fused with a polyhistidine tag at the C-terminus.
Protein Construction: A DNA sequence encoding the mature form of human NRN1 (Q9NPD7) (Met 1-Asn 115) was fused with a polyhistidine tag at the C-terminus.
Fusion Tag: C-His
Species: Human
Expressed Host: Baculovirus-Insect Cells
Shipping: This product is provided as lyophilized powder which is shipped with ice packs.
Purity: > 85 % as determined by reducing SDS-PAGE.
Endotoxin: < 1.0 EU per µg of the protein as determined by the LAL method.
Stability and Storage: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.
Molecular Mass: N/A
Formulation: Lyophilized from sterile 20mM Tris, 500mM NaCl, pH 8.0, 10% gly
Reconstitution: Please refer to the printed manual for detailed information.
Background: Neuritin 1 (NRN1) is a member of neuritin family. Neuritin is a glycosylphosphatidylinositol- anchored protein induced by neural activity. It is expressed in postmitotic-differentiating neurons of the developing nervous system and a population of small-diameter neurons in the dorsal root ganglia and was anterogradely and retrogradely transported. Neuritin message is induced by neuronal activity and by the activity-regulated neurotrophins BDNF; nerve growth factor (NGF) and NT-3. Purified recombinant neuritin promotes neurite outgrowth and arborization in primary embryonic hippocampal and cortical cultures. Thus; neuritin is considered as a downstream effector of activity-induced neurite outgrowth. In clinical; neuritin levels in diabetes were reduced in both dorsal root ganglia and sciatic nerve of rats; and these deficits were reversed in vivo by treatment with NGF. This manipulation of neuritin levels in diabetes may provide a potential target for the therapeutic intervention in the management of neuropathy.
Research Area: Neuroscience
