Description
Recombinant Human NCK1 Protein (His Tag) | PKSH032339 | Gentaur US, UK & Europe Disrtribition
Synonyms: Cytoplasmic Protein NCK1; NCK adaptor Protein 1; Nck-1; SH2/SH3 Adaptor Protein NCK-Alpha; NCK1; NCK
Active Protein: N/A
Activity: Recombinant Human Non-catalytic Region of Tyrosine Kinase Adaptor Protein 1 is produced by our E.coli expression system and the target gene encoding Met1-Ser377 is expressed with a 6His tag at the N-terminus.
Protein Construction: Recombinant Human Non-catalytic Region of Tyrosine Kinase Adaptor Protein 1 is produced by our E.coli expression system and the target gene encoding Met1-Ser377 is expressed with a 6His tag at the N-terminus.
Fusion Tag: N-6His
Species: Human
Expressed Host: E.coli
Shipping: This product is provided as lyophilized powder which is shipped with ice packs.
Purity: > 80 % as determined by reducing SDS-PAGE.
Endotoxin: < 1.0 EU per µg as determined by the LAL method.
Stability and Storage: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.
Molecular Mass: 45.0 kDa
Formulation: Lyophilized from a 0.2 μm filtered solution of 20mM Tris, 150mM NaCl, 5% Mannitol, pH 8.0.
Reconstitution: Please refer to the printed manual for detailed information.
Background: Cytoplasmic Protein NCK1 (NCK1) is a cytoplasmic protein that contains one SH2 domain and three SH3 domains. NCK1 is a member of the adapter family, which associates with tyrosine-phosphorylated growth factor receptors, such as KDR and PDGFRB, or their cellular substrates. NCK1 maintains low levels of EIF2S1 phosphorylation by promoting its dephosphorylation by PP1. NCK1 plays a role in the DNA damage response, but not in the detection of the damage by ATM/ATR. It is also involved in transducing signals from receptor tyrosine kinases to downstream signal recipients, such as ELK1-dependent transcriptional activation in response to activated Ras signaling.
Research Area: Signal Transduction, Cancer, epigenetics and nuclear signal,
