Description
Recombinant Human N-Glycosylase/OGG1 Protein (GST Tag) | PKSH032809 | Gentaur US, UK & Europe Disrtribition
Synonyms: N-Glycosylase/DNA Lyase; 8-Oxoguanine DNA Glycosylase; DNA-(Apurinic or Apyrimidinic Site) Lyase; AP Lyase; OGG1; MMH; MUTM; OGH1
Active Protein: N/A
Activity: Recombinant Human N-Glycosylase is produced by our E.coli expression system and the target gene encoding Met1-Gly345 is expressed with a GST tag at the N-terminus.
Protein Construction: Recombinant Human N-Glycosylase is produced by our E.coli expression system and the target gene encoding Met1-Gly345 is expressed with a GST tag at the N-terminus.
Fusion Tag: N-GST
Species: Human
Expressed Host: E.coli
Shipping: This product is provided as liquid. It is shipped at frozen temperature with blue ice/gel packs. Upon receipt, store it immediately at<-20°C.
Purity: > 95 % as determined by reducing SDS-PAGE.
Endotoxin: < 1.0 EU per µg as determined by the LAL method.
Stability and Storage: Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles.
Molecular Mass: 65.1 kDa
Formulation: Supplied as a 0.2 μm filtered solution of 20mM TrisHCl, 100mM NaCl, 1mM DTT, 1mM EDTA, 50% Glycerol, pH 7.8.
Reconstitution: Not Applicable
Background: Human N-Glycosylase/DNA Lyase(OOG1) is a DNA repair enzyme, which belongs to the type-1 OGG1 family. OOG1 incises DNA at 8-oxoG residues, and excises 7, 8-dihydro-8-oxoguanine and 2, 6-diamino-4-hydroxy-5-N-methylformamidopyrimidine (FAPY) from damage DNA. It has a β-lyase activity that nicks DNA 3’ to the lesion. OOG1 together with APEX1 is recruited to nuclear speckles in UVA-irradiated cells. The OGG1 gene mutations may be caused Renal cell carcinoma.
Research Area: Cancer, epigenetics and nuclear signal
