Recombinant Human MMP1 Protein (His Tag)(Active) | PKSH031542

Recombinant Human MMP1 Protein (His Tag)(Active) | PKSH031542 | Gentaur US, UK & Europe Disrtribition

Synonyms: Interstitial Collagenase; Fibroblast Collagenase; Matrix Metalloproteinase-1; MMP-1; MMP1; CLG

Active Protein: Active protein

Activity: A DNA sequence encoding the pro form of human MMP1 enzyme (NP_002412.1) precursor (Met 1-Asn 469) was expressed with a C-terminal polyhistidine tag.

Protein Construction: A DNA sequence encoding the pro form of human MMP1 enzyme (NP_002412.1) precursor (Met 1-Asn 469) was expressed with a C-terminal polyhistidine tag.

Fusion Tag: C-His

Species: Human

Expressed Host: HEK293 Cells

Shipping: This product is provided as lyophilized powder which is shipped with ice packs.

Purity: > 96 % as determined by reducing SDS-PAGE.

Endotoxin: < 1.0 EU per µg as determined by the LAL method.

Stability and Storage: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.

Molecular Mass: N/A

Formulation: Lyophilized from sterile 25mM MES, 10mM CaCl2, 150mM NaCl, 0.05% Brij 35, pH 5.5

Reconstitution: Please refer to the printed manual for detailed information.

Background: MMP1, also known as MMP-1, contains 4 hemopexin-like domains and is a member of the matrix metalloproteinase (MMP) family. Matrix metalloproteases, also called matrixins, are zinc-dependent endopeptidases that are the major proteases involved in ECM degradation. MMPs are capable of degrading a wide range of extracellular molecules and a number of bioactive molecules. MMP activity is regulated by two major endogenous inhibitors: alpha2-macroglobulin and tissue inhibitors of metalloproteases (TIMPs). MMPs play a central role in cell proliferation, migration, differentiation, angiogenesis, apoptosis and host defences. Dysregulatoin of MMPs has been implicated in many diseases including arthritis, chronic ulcers, encephalomyelitis and cancer. Tumour metastasis is a multistep process involving the dessemination of tumor cells from the primary tumor to secondarys at a distant organ or tissue. One of the first steps in metastasis is the degradation of the basement membrane, a process in which MMPs have been implicated. MMPs are secreted by tumor cells themselves or by surrounding stromal cells stimulated by the nearby tumor. Numerous studies have linked altered MMP expression in different human cancers with poor disease prognosis. MMP-1, -2, -3, -7, -9, -13 and -14 all have elevated expression in primary tumors and/or metastases. MMP-1 cleaves collagens of types I, II, and III at one site in the helical domain. It also cleaves collagens of types VII and X. In case of HIV infection, MMP1 interacts and cleaves the secreted viral Tat protein, leading to a decrease in neuronal Tat's mediated neurotoxicity.

Research Area: Signal Transduction, Cell biology, Cardiovascular, Cancer,