Recombinant Human METTL1 Protein (His Tag) | PKSH031101

Recombinant Human METTL1 Protein (His Tag) | PKSH031101 | Gentaur US, UK & Europe Disrtribition

Synonyms: C12orf1;TRM8;TRMT8;YDL201w

Active Protein: N/A

Activity: A DNA sequence encoding the human METTL1 (NP_005362.3) (Asp 32-Gln 265) was expressed, with a polyhistide tag at the N-terminus.

Protein Construction: A DNA sequence encoding the human METTL1 (NP_005362.3) (Asp 32-Gln 265) was expressed, with a polyhistide tag at the N-terminus.

Fusion Tag: N-His

Species: Human

Expressed Host: E.coli

Shipping: This product is provided as lyophilized powder which is shipped with ice packs.

Purity: > 95 % as determined by reducing SDS-PAGE.

Endotoxin: Please contact us for more information.

Stability and Storage: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.

Molecular Mass: 28 kDa

Formulation: Lyophilized from sterile 50mM Tris, 0.5M NaCl, 20% glycerol, pH 8.0

Reconstitution: Please refer to the printed manual for detailed information.

Background: tRNA (guanine-N(7)-)-methyltransferase, also known as Methyltransferase-like protein 1, tRNA (m7G46)-methyltransferase and METTL1, is a nucleus protein which belongs to the methyltransferase superfamily and TrmB family. METTL1 gene, has been identified by its sequence similarity to the yeast ORF YDL201w. The human cDNA and the genomic structure of METTL1 have been analyzed. The transcript contains 1292 nucleotides and codes for a protein of 276 amino acids. The METTL1 gene product shows high sequence similarities to putative proteins from mouse, Drosophila melanogaster, Arabidopsis thaliana, Caenorhabditis elegans, and yeast (39.8% identity between all six species). Computer analyses of the deduced protein sequence reveal two highly conserved amino acid motifs, one of which is typical for methyltransferases. Both motifs are also present in hypothetical proteins from eubacteria. Disruption of the homologous yeast ORF YDL201w shows that the gene is at least not essential for vegetative growth in Saccharomyces cerevisiae.

Research Area: epigenetics and nuclear signal