Recombinant Human Legumain/LGMN Protein (His Tag) | PKSH032687

Recombinant Human Legumain/LGMN Protein (His Tag) | PKSH032687 | Gentaur US, UK & Europe Disrtribition

Synonyms: Legumain; Asparaginyl Endopeptidase; Protease Cysteine 1; LGMN; PRSC1

Active Protein: N/A

Activity: Recombinant Human Legumain/Asparaginyl Endopeptidase is produced by our Mammalian expression system and the target gene encoding Ile18-Tyr433 is expressed with a 6His tag at the C-terminus.

Protein Construction: Recombinant Human Legumain/Asparaginyl Endopeptidase is produced by our Mammalian expression system and the target gene encoding Ile18-Tyr433 is expressed with a 6His tag at the C-terminus.

Fusion Tag: C-6His

Species: Human

Expressed Host: Human Cells

Shipping: This product is provided as liquid. It is shipped at frozen temperature with blue ice/gel packs. Upon receipt, store it immediately at<-20°C.

Purity: > 90 % as determined by reducing SDS-PAGE.

Endotoxin: < 1.0 EU per µg as determined by the LAL method.

Stability and Storage: Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles.

Molecular Mass: 48.7 kDa

Formulation: Supplied as a 0.2 μm filtered solution of 20mM TrisHCl, 150mM NaCl, 10% Glycerol, pH 8.0.

Reconstitution: Not Applicable

Background: Legumain is a lysosomal cysteine protease which is a member of the peptidase C13 family. Though it is found in many tissues, it is highly expressed in the kidney, heart, and placenta. Legumain has a strict specificity for hydrolysis of asparaginyl bonds and can also cleave aspartyl bonds slowly, especially under acidic conditions. Over-expression Legumain in tumors is significant for invasion and metastasis. In addition, Legumain may be involved in the processing of proteins for MHC class II antigen presentation in the lysosomal/endosomal system and negative regulation of neuron apoptosis.

Research Area: Signal Transduction, Cell biology, Cancer, immunology, metabolism,