Recombinant Human LCP2 Protein (His Tag, N-T7 Tag) | PKSH032716

Recombinant Human LCP2 Protein (His Tag, N-T7 Tag) | PKSH032716 | Gentaur US, UK & Europe Disrtribition

Synonyms: Lymphocyte cytosolic protein 2;SH2 domain-containing leukocyte protein of 76 kDa;SLP-76 tyrosine phosphoprotein;SLP76;LCP2

Active Protein: N/A

Activity: Recombinant Human Lymphocyte cytosolic protein 2/SH2 Domain-containing Leukocyte Protein of 76 kDa is produced by our E.coli expression system and the target gene encoding Met1-Pro533 is expressed with a T7 tag at the N-terminus, 6His tag at the C-terminus.

Protein Construction: Recombinant Human Lymphocyte cytosolic protein 2/SH2 Domain-containing Leukocyte Protein of 76 kDa is produced by our E.coli expression system and the target gene encoding Met1-Pro533 is expressed with a T7 tag at the N-terminus, 6His tag at the C-terminus.

Fusion Tag: C-6His

Species: Human

Expressed Host: E.coli

Shipping: This product is provided as liquid. It is shipped at frozen temperature with blue ice/gel packs. Upon receipt, store it immediately at<-20°C.

Purity: > 95 % as determined by reducing SDS-PAGE.

Endotoxin: < 1.0 EU per µg as determined by the LAL method.

Stability and Storage: Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles.

Molecular Mass: 62.6 kDa

Formulation: Supplied as a 0.2 μm filtered solution of 20mM Tris, 150mM NaCl, 20% glycerol，pH 8.5.

Reconstitution: Not Applicable

Background: Lymphocyte cytosolic protein 2（LCP2）contains a SAM domain and a SH2 domain. It is highly expressed in spleen, thymus and peripheral blood leukocytes, T-cell and monocytic cell lines, but expressed at lower level in B-cell lines. LCP2 was originally identified as a substrate of the ZAP-70 protein tyrosine kinase following T cell receptor (TCR) ligation in the leukemic T cell line Jurkat. It is phosphorylated after T-cell receptor activation by ZAP70, ITK and TXK, which leads to the up-regulation of Th1 preferred cytokine IL-2 during post-translational modification. Studies using LCP2-deficient T cell lines or mice have provided strong evidence that SLP-76 plays a positive role in promoting T cell development and activation as well as mast cell and platelet function.

Research Area: Signal Transduction,