Recombinant Human IgG4-Fc Protein (aa 104-327, Leu325Pro) | PKSH033651

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SKU:
575-PKSH033651
Weight:
1.00 KGS
€519.00
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Description

Recombinant Human IgG4-Fc Protein (aa 104-327, Leu325Pro) | PKSH033651 | Gentaur US, UK & Europe Disrtribition

Synonyms: Ig gamma-4 chain C region, IgG4 Fc;IGHG4

Active Protein: N/A

Activity: Recombinant Human IgG4-Fc is produced by our Mammalian expression system and the target gene encoding Pro104-Lys327(Leu325Pro) is expressed.

Protein Construction: Recombinant Human IgG4-Fc is produced by our Mammalian expression system and the target gene encoding Pro104-Lys327(Leu325Pro) is expressed.

Fusion Tag: N/A

Species: Human

Expressed Host: Human Cells

Shipping: This product is provided as lyophilized powder which is shipped with ice packs.

Purity: > 95 % as determined by reducing SDS-PAGE.

Endotoxin: < 1.0 EU per μg as determined by the LAL method.

Stability and Storage: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.

Molecular Mass: 25.1 kDa

Formulation: Lyophilized from a 0.2 μm filtered solution of 20mM PB, 150mM NaCl, pH7.4.

Reconstitution: Please refer to the printed manual for detailed information.

Background: As a monomeric immunoglobulin that is predominately involved in the secondary antibody response and the only isotype that can pass through the human placenta; Immunoglobulin G (IgG) is synthesized and secreted by plasma B cells; and constitutes 75% of serum immunoglobulins in humans. IgG antibodies protect the body against the pathogens by agglutination and immobilization; complement activation; toxin neutralization; as well as the antibody-dependent cell-mediated cytotoxicity (ADCC). IgG tetramer contains two heavy chains (50 kDa ) and two light chains (25 kDa) linked by disulfide bonds; that is the two identical halves form the Y-like shape. IgG is digested by pepsin proteolysis into Fab fragment (antigen-binding fragment) and Fc fragment ("crystallizable" fragment). IgG1 is most abundant in serum among the four IgG subclasses (IgG1; 2; 3 and 4) and binds to Fc receptors (FcγR ) on phagocytic cells with high affinity. Fc fragment is demonstrated to mediate phagocytosis; trigger inflammation; and target Ig to particular tissues. Protein G or Protein A on the surface of certain Staphylococcal and Streptococcal strains specifically binds with the Fc region of IgGs; and has numerous applications in biotechnology as a reagent for affinity purification. Recombinant IgG Fc Region is suggested to represent a potential anti-inflammatory drug for treatment of human autoimmune diseases.

Research Area: immunology

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