Description
Recombinant Human IgG1 Fc Biotinylated | PKSH034027 | Gentaur US, UK & Europe Disrtribition
Synonyms: g gamma-1 chain C region; IGHG1
Active Protein: N/A
Activity: Biotinylated Recombinant Human Immunoglobulin G1 Fc is produced by our Mammalian expression system and the target gene encoding Asp104-Lys330(Asp239Glu, Leu241Glu) is expressed with a Avi tag at the C-terminus.
Protein Construction: Biotinylated Recombinant Human Immunoglobulin G1 Fc is produced by our Mammalian expression system and the target gene encoding Asp104-Lys330(Asp239Glu, Leu241Glu) is expressed with a Avi tag at the C-terminus.
Fusion Tag: C-Avi
Species: Human
Expressed Host: Human Cells
Shipping: This product is provided as lyophilized powder which is shipped with ice packs.
Purity: > 95 % as determined by reducing SDS-PAGE.
Endotoxin: < 1.0 EU per µg as determined by the LAL method.
Stability and Storage: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.
Molecular Mass: 27.7 kDa
Formulation: Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.
Reconstitution: Please refer to the printed manual for detailed information.
Background: As a monomeric immunoglobulin that is predominately involved in the secondary antibody response and the only isotype that can pass through the human placenta, Immunoglobulin G (IgG) is synthesized and secreted by plasma B cells, and constitutes 75% of serum immunoglobulins in humans. IgG antibodies protect the body against the pathogens by agglutination and immobilization, complement activation, toxin neutralization, as well as the antibody-dependent cell-mediated cytotoxicity (ADCC). IgG tetramer contains two heavy chains (50 kDa ) and two light chains (25 kDa) linked by disulfide bonds, that is the two identical halves form the Y-like shape. IgG is digested by pepsin proteolysis into Fab fragment (antigen-binding fragment) and Fc fragment ("crystallizable" fragment). IgG1 is most abundant in serum among the four IgG subclasses (IgG1, 2, 3 and 4) and binds to Fc receptors (FcγR ) on phagocytic cells with high affinity.
Research Area: immunology
