Description
Recombinant Human IDH1 Protein (R132H, C-8His) | PKSH032660 | Gentaur US, UK & Europe Disrtribition
Synonyms: Isocitrate Dehydrogenase [NADP] Cytoplasmic; IDH; Cytosolic NADP-Isocitrate Dehydrogenase; IDP; NADP(+)-Specific ICDH; Oxalosuccinate Decarboxylase; IDH1; PICD
Active Protein: N/A
Activity: Recombinant Human Isocitrate Dehydrogenase [NADP] Cytoplasmic is produced by our E.coli expression system and the target gene encoding Met1-Leu414(Arg132His) is expressed with a 8His tag at the C-terminus.
Protein Construction: Recombinant Human Isocitrate Dehydrogenase [NADP] Cytoplasmic is produced by our E.coli expression system and the target gene encoding Met1-Leu414(Arg132His) is expressed with a 8His tag at the C-terminus.
Fusion Tag: C-8His
Species: Human
Expressed Host: E.coli
Shipping: This product is provided as liquid. It is shipped at frozen temperature with blue ice/gel packs. Upon receipt, store it immediately at<-20°C.
Purity: > 95 % as determined by reducing SDS-PAGE.
Endotoxin: < 1.0 EU per µg as determined by the LAL method.
Stability and Storage: Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles.
Molecular Mass: 48.1 kDa
Formulation: Supplied as a 0.2 μm filtered solution of 50mM Tris, 200mM NaCl, 10% glycerol, pH8.0.
Reconstitution: Not Applicable
Background: Isocitrate Dehydrogenase [NADP] Cytoplasmic (IDH1) belongs to the isocitrate and isopropylmalate dehydrogenases family. IDH1 exists as a homodimer; binding one magnesium or manganese ion per subunit. Mutations of IDH1 have been shown to cause metaphyseal chondromatosis with aciduria and are involved in the development of glioma. IDH plays a role in the regeneration of NADPH for intraperoxisomal reductions; such as the conversion of 2; 4-dienoyl-CoAs to 3-enoyl-CoAs; as well as in peroxisomal reactions that consume 2-oxoglutarate; namely the α-hydroxylation of phytanic acid.
Research Area: Signal Transduction, Cancer, metabolism,
