Description
Recombinant Human IDE/Insulysin Protein (His Tag) | PKSH032593 | Gentaur US, UK & Europe Disrtribition
Synonyms: Insulin-Degrading Enzyme; Abeta-Degrading Protease; Insulin Protease; Insulinase; Insulysin; IDE
Active Protein: N/A
Activity: Recombinant Human Insulin-Degrading Enzyme is produced by our Mammalian expression system and the target gene encoding Met42-Leu1019 is expressed with a 6His tag at the C-terminus.
Protein Construction: Recombinant Human Insulin-Degrading Enzyme is produced by our Mammalian expression system and the target gene encoding Met42-Leu1019 is expressed with a 6His tag at the C-terminus.
Fusion Tag: C-6His
Species: Human
Expressed Host: Human Cells
Shipping: This product is provided as liquid. It is shipped at frozen temperature with blue ice/gel packs. Upon receipt, store it immediately at<-20°C.
Purity: > 95 % as determined by reducing SDS-PAGE.
Endotoxin: < 1.0 EU per µg as determined by the LAL method.
Stability and Storage: Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles.
Molecular Mass: 114.3 kDa
Formulation: Supplied as a 0.2 μm filtered solution of 20mM TrisHCl, 150mM NaCl, 0.05%Brij35, 10%Glycerol, pH7.5.
Reconstitution: Not Applicable
Background: Insulin-Degrading Enzyme (IDE) is a secreted enzyme that belongs to the peptidase M16 family. IDE is a large zinc-binding protease and cleaves multiple short polypeptides that vary considerably in sequence. IDE plays a role in the cellular breakdown of insulin, IAPP, glucagon, bradykinin, kallidin, and other peptides, and thereby plays a role in intercellular peptide signaling. IDE degrades amyloid formed by APP and IAPP. IDE may participate in the degradation and clearance of naturally secreted amyloid β-protein by neurons and microglia. IDE, which migrates at 110 kDa during gel electrophoresis under denaturing conditions, has since been shown to have additional substrates, including the signaling peptides glucagon, TGF α and β-endorphin.
Research Area: Signal Transduction, Cell biology, Cardiovascular, Neuroscience, Cancer, metabolism,
