Recombinant Human FLT-3/FLK-2 Protein (Fc Tag) | PKSH033543

Recombinant Human FLT-3/FLK-2 Protein (Fc Tag) | PKSH033543 | Gentaur US, UK & Europe Disrtribition

Synonyms: Receptor-Type Tyrosine-Protein Kinase FLT3; FL Cytokine Receptor; Fetal Liver Kinase-2; FLK-2; Fms-Like Tyrosine Kinase 3; FLT-3; Stem Cell Tyrosine Kinase 1; STK-1; CD135; FLT3; FLK2; STK1

Active Protein: N/A

Activity: Recombinant Human FLT-3 is produced by our Mammalian expression system and the target gene encoding Asn27-Asn541(Thr227Met) is expressed with a Fc tag at the C-terminus.

Protein Construction: Recombinant Human FLT-3 is produced by our Mammalian expression system and the target gene encoding Asn27-Asn541(Thr227Met) is expressed with a Fc tag at the C-terminus.

Fusion Tag: C-Fc

Species: Human

Expressed Host: Human Cells

Shipping: This product is provided as liquid. It is shipped at frozen temperature with blue ice/gel packs. Upon receipt, store it immediately at<-20°C.

Purity: > 95 % as determined by reducing SDS-PAGE.

Endotoxin: < 1.0 EU per μg as determined by the LAL method.

Stability and Storage: Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles.

Molecular Mass: 85.3 kDa

Formulation: Supplied as a 0.2 μm filtered solution of PBS, PH7.4.

Reconstitution: Not Applicable

Background: The Flt-3 (fms-like tyrosine kinase) receptor, also named Flk-2and Stk-1is a member of the class III subfamily of receptor tyrosine kinases that also includes KIT, the receptor for SCF and FMS, the receptor for M-CSF. The extracellular region of these receptors contains five immunoglobulin-like domains and the intracellular region contains a split kinase domain. Human Flt-3 cDNA encodes a 993 amino acid (aa) residue type I membrane protein with a 26 aa residue signal peptide, a 515 aa extracellular domain with 10 potential N-linked glycosylation sites, a 21 aa residue transmembrane domain and a 431 aa residue cytoplasmic domain. Flt-3 expression has been detected in various tissues, including placenta, gonads, and tissues of nervous and hematopoietic origin. Among hematopoietic cells, the expression of Flt-3 was found to be restricted to the highly enriched stem/progenitor cell populations. The ligand for Flt-3 (FL) has been identified to be a transmembrane protein with structural homology to M-CSF and SCF. Recombinant soluble Flt-3/Fc chimeric protein has been shown to bind FL with high affinity and is a potent FL antagonist.

Research Area: Signal Transduction, immunology,