Recombinant Human Flap Endonuclease 1/FEN1 Protein | PKSH032451

Recombinant Human Flap Endonuclease 1/FEN1 Protein | PKSH032451 | Gentaur US, UK & Europe Disrtribition

Synonyms: Flap Endonuclease 1; FEN-1; DNase IV; Flap Structure-Specific Endonuclease 1; Maturation Factor 1; MF1; hFEN-1; FEN1; RAD2

Active Protein: N/A

Activity: Recombinant Human Flap Endonuclease 1 is produced by our E.coli expression system and the target gene encoding Met1-Lys380 is expressed.

Protein Construction: Recombinant Human Flap Endonuclease 1 is produced by our E.coli expression system and the target gene encoding Met1-Lys380 is expressed.

Fusion Tag:

Species: Human

Expressed Host: E.coli

Shipping: This product is provided as liquid. It is shipped at frozen temperature with blue ice/gel packs. Upon receipt, store it immediately at<-20°C.

Purity: > 90 % as determined by reducing SDS-PAGE.

Endotoxin: < 1.0 EU per µg as determined by the LAL method.

Stability and Storage: Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles.

Molecular Mass: 42.6 kDa

Formulation: Supplied as a 0.2 μm filtered solution of 50 mM Tris, 50mM NaCl, 1mM DDT, 10% Glycerol, pH 8.0.

Reconstitution: Not Applicable

Background: Flap Endonuclease 1 (FEN1) is a member of the XPG/RAD2 endonuclease family. During DNA replication, FEN1 cleaves the 5'-overhanging flap structure and processes the 5' ends of Okazaki fragments for synthesis. FEN1 also exhibits RNase H activity by possessing 5'-3' exonuclease activity on gapped double-stranded or nicked DNA, FEN1 is involved in the long patch base excision repair (LP-BER) pathway, it can cleave within the apurinic/apyrimidinic (AP) site-terminated flap. FEN1 can prevent flaps from equilibrating into structures that lead to duplications and deletions. FEN1 is also involved in replication and repair of rDNA and in repairing mitochondrial DNA.

Research Area: epigenetics and nuclear signal