Recombinant Human F13a/Factor XIIIa Protein (His Tag) | PKSH033713

Recombinant Human F13a/Factor XIIIa Protein (His Tag) | PKSH033713 | Gentaur US, UK & Europe Disrtribition

Synonyms: Coagulation Factor XIII A Chain; Coagulation Factor XIIIa; Protein-Glutamine Gamma-Glutamyltransferase A Chain; Transglutaminase A Chain; F13A1; F13A

Active Protein: N/A

Activity: Recombinant Human Coagulation Factor XIII A Chain is produced by our Mammalian expression system and the target gene encoding Gly39-Met732 is expressed with a 6His tag at the C-terminus.

Protein Construction: Recombinant Human Coagulation Factor XIII A Chain is produced by our Mammalian expression system and the target gene encoding Gly39-Met732 is expressed with a 6His tag at the C-terminus.

Fusion Tag: C-His

Species: Human

Expressed Host: Human Cells

Shipping: This product is provided as liquid. It is shipped at frozen temperature with blue ice/gel packs. Upon receipt, store it immediately at<-20°C.

Purity: > 95 % as determined by reducing SDS-PAGE.

Endotoxin: < 1.0 EU per μg as determined by the LAL method.

Stability and Storage: Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles.

Molecular Mass: 80.3 kDa

Formulation: Supplied as a 0.2 μm filtered solution of 50 mM NaCl, 5% Sucrose, 1% Tween 20 (v/v), 0.3% Histidine (w/v), pH8.0.

Reconstitution: Not Applicable

Background: Coagulation factor XIII is the last zymogen to become activated in the blood coagulation cascade. Plasma factor XIII is a heterotetramer composed of 2 A subunits and 2 B subunits. The A subunits have catalytic function, and the B subunits do not have enzymatic activity and may serve as plasma carrier molecules. Platelet factor XIII is composed of just 2 A subunits, which are identical to those of plasma origin. Upon cleavage of the activation peptide by thrombin and in the presence of calcium ion, the plasma factor XIII dissociates its B subunits and yields the same active enzyme, factor XIIIa, as platelet factor XIII. This enzyme acts as a transglutaminase to catalyze the formation of gamma-glutamyl-epsilon-lysine crosslinking between fibrin molecules, thus stabilizing the fibrin clot. Factor XIII deficiency is classified into two categories: type I deficiency, characterized by the lack of both the A and B subunits; and type II deficiency, characterized by the lack of the A subunit alone. These defects can result in a lifelong bleeding tendency, defective wound healing, and habitual abortion.

Research Area: Tags & Cell Markers, Cardiovascular