Recombinant Human Ezrin/EZR Protein | PKSH033684

Recombinant Human Ezrin/EZR Protein | PKSH033684 | Gentaur US, UK & Europe Disrtribition

Synonyms: Ezrin; Cytovillin; Villin-2; p81; EZR; VIL2

Active Protein: N/A

Activity: Recombinant Human Ezrin is produced by our E.coli expression system and the target gene encoding Met1-Leu586 is expressed.

Protein Construction: Recombinant Human Ezrin is produced by our E.coli expression system and the target gene encoding Met1-Leu586 is expressed.

Fusion Tag: N/A

Species: Human

Expressed Host: E.coli

Shipping: This product is provided as lyophilized powder which is shipped with ice packs.

Purity: > 95 % as determined by reducing SDS-PAGE.

Endotoxin: < 1.0 EU per μg as determined by the LAL method.

Stability and Storage: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.

Molecular Mass: 69.4 kDa

Formulation: Lyophilized from a 0.2 μm filtered solution of 10mM Hepes, pH7.4.

Reconstitution: Please refer to the printed manual for detailed information.

Background: Ezrin is expressed in cerebral cortex, basal ganglia, hippocampus, hypophysis, and optic nerve. The N-terminus of ezrin contains a FERM domain which is further subdivided into three subdomains. The C-terminus contain a ERM domain. As a member of the ERM protein family, Ezrin serves as an intermediate between the plasma membrane and the actin cytoskeleton. It plays a key role in cell surface structure adhesion, migration, and organization. Ezrin probably involved in connections of major cytoskeletal structures to the plasma membrane. The N-terminal FERM domain strongly binds sodium-hydrogen exchanger regulatory factor (NHERF) proteins (involving long-range allostery). The C-terminal binds to actin, phosphatidylinositol bis-phosphate (PIP2) and membrane proteins like CD44 and ICAM-2. In epithelial cells, Ezrin is required for the formation of microvilli and membrane ruffles on the apical pole. Along with PLEKHG6, Ezrin is required for normal macropinocytosis.

Research Area: Signal Transduction, Cancer,