Description
Recombinant Human ESAM Protein (aa 30-247, Fc Tag) | PKSH032380 | Gentaur US, UK & Europe Disrtribition
Synonyms: Endothelial Cell-Selective Adhesion Molecule; ESAM
Active Protein: N/A
Activity: Recombinant Human Endothelial Cell Adhesion Molecule is produced by our Mammalian expression system and the target gene encoding Gln30-Ala247 is expressed with a Fc tag at the C-terminus.
Protein Construction: Recombinant Human Endothelial Cell Adhesion Molecule is produced by our Mammalian expression system and the target gene encoding Gln30-Ala247 is expressed with a Fc tag at the C-terminus.
Fusion Tag: C-Fc
Species: Human
Expressed Host: Human Cells
Shipping: This product is provided as lyophilized powder which is shipped with ice packs.
Purity: > 95 % as determined by reducing SDS-PAGE.
Endotoxin: < 1.0 EU per µg as determined by the LAL method.
Stability and Storage: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.
Molecular Mass: 50.8 kDa
Formulation: Lyophilized from a 0.2 μm filtered solution of 20mM PB, 150mM NaCl, pH7.4.
Reconstitution: Please refer to the printed manual for detailed information.
Background: Endothelial Cell Adhesion Molecule (ESAM) is a 55 kDa type I transmembrane glycoprotein member of the JAM family of immunoglobulin superfamily molecules. The 390 amino acid Human ESAM contains a 216 amino acid extracellular domain (ECD) with a V-type and a C2-type immunoglobulin (Ig) domain. The ECD of human and mouse ESAM share 69% amino acid identity. ESAM is specifically expressed at endothelial tight junctions and on activated platelets and performs homophilic adhesion activity. The adaptor protein membrane-associated guanylate kinase MAGI-1 has been identified as an intracellular binding partner of ESAM. In addition; ESAM at endothelial tight junctions participates in the migration of neutrophils through the vessel wall; possibly by influencing endothelial cell contacts. ESAM-deficient mice were described with lowered angiogenic potential; and accordingly; overexpression of ESAM is closely associated with certain tumor growth and metastasis.
Research Area: Signal Transduction, Tags & Cell Markers, Cardiovascular, Cancer,
