Description
Recombinant Human EphA4 Protein (aa 570-986, His &GST Tag)(Active) | PKSH030369 | Gentaur US, UK & Europe Disrtribition
Synonyms: Ephrin type-A receptor 4;HEK8; SEK; TYRO1;EPHA4;Tyrosine-protein kinase receptor SEK;Tyrosine-protein kinase TYRO1;EK8;hEK8;EPH-like kinase 8
Active Protein: Active protein
Activity: A DNA sequence encoding the human EPHA4 (P54764) (Ser570-Val986) was fused with the N-terminal polyhistidine-tagged GST tag at the N-terminus.
Protein Construction: A DNA sequence encoding the human EPHA4 (P54764) (Ser570-Val986) was fused with the N-terminal polyhistidine-tagged GST tag at the N-terminus.
Fusion Tag: N-His-GST
Species: Human
Expressed Host: Baculovirus-Insect Cells
Shipping: This product is provided as liquid. It is shipped at frozen temperature with blue ice/gel packs. Upon receipt, store it immediately at<-20°C.
Purity: > 99 % as determined by reducing SDS-PAGE.
Endotoxin: < 1.0 EU per µg of the protein as determined by the LAL method.
Stability and Storage: Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles.
Molecular Mass: 75 kDa
Formulation: Supplied as sterile 20mM Tris, 500mM NaCl, pH 8.5, 10% glycerol, 3mM DTT
Reconstitution: Not Applicable
Background: EPH receptor A4 (ephrin type-A receptor 4); also known as EphA4; belongs to the ephrin receptor subfamily of the protein-tyrosine kinase family which 16 known receptors (14 found in mammals) are involved: EPHA1; EPHA2; EPHA3; EPHA4; EPHA5; EPHA6; EPHA7; EPHA8; EPHA9; EPHA10; EPHB1; EPHB2; EPHB3; EPHB4; EPHB5; EPHB6. The Eph family of receptor tyrosine kinases (comprising EphA and EphB receptors) has been implicated in synapse formation and the regulation of synaptic function and plasticity6. EphA4 is enriched on dendritic spines of pyramidal neurons in the adult mouse hippocampus; and ephrin-A3 is localized on astrocytic processes that envelop spines. Eph receptor−mediated signaling; which is triggered by ephrins7; probably modifies the properties of synapses during synaptic activation and remodeling. Ephrin receptors are components of cell signalling pathways involved in animal growth and development; forming the largest sub-family of receptor tyrosine kinases (RTKs). The extracellular domain of an EphA4 interacts with ephrin ligands; which may be tethered to neighbouring cells. Ligand-mediated activation of Ephs induce various important downstream effects and Eph receptors have been studied for their potential roles in the development of cancer.
Research Area: Signal Transduction, Cardiovascular, Neuroscience,
