Description
Recombinant Human ENO1/Enolase 1/alpha-enolase Protein (His Tag) | PKSH031092 | Gentaur US, UK & Europe Disrtribition
Synonyms: ENO1L1;HEL-S-17;MPB1;NNE;PPH
Active Protein: N/A
Activity: A DNA sequence encoding the human ENO1 (P06733-1) (Met 1-Lys 434) was expressed, with a polyhistide tag at the N-terminus.
Protein Construction: A DNA sequence encoding the human ENO1 (P06733-1) (Met 1-Lys 434) was expressed, with a polyhistide tag at the N-terminus.
Fusion Tag: N-His
Species: Human
Expressed Host: E.coli
Shipping: This product is provided as lyophilized powder which is shipped with ice packs.
Purity: > 95 % as determined by reducing SDS-PAGE.
Endotoxin: Please contact us for more information.
Stability and Storage: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.
Molecular Mass: 49.3 kDa
Formulation: Lyophilized from sterile 20mM Tris, 10% glycerol, pH 8.5
Reconstitution: Please refer to the printed manual for detailed information.
Background: Alpha-enolase, also known as MPB-1, NNE, Enolase 1, belongs to the enolase family. Mammalian enolase is composed of 3 isozyme subunits, alpha, beta and gamma, which can form homodimers or heterodimers which are cell-type and development-specific. ENO1 interacts with PLG in the neuronal plasma membrane and promotes its activation. The C-terminal lysine is required for this binding. Isoform MBP-1 interacts with TRAPPC2B. ENO1 interacts with ENO4 and PGAM2. The alpha/alpha homodimer of ENO1 is expressed in embryo and in most adult tissues. The alpha/beta heterodimer and the beta/beta homodimer are found in striated muscle, and the alpha/gamma heterodimer and the gamma/gamma homodimer in neurons. During ontogenesis, there is a transition from the alpha/alpha homodimer to the alpha/beta heterodimerin striated muscle cells, and to the alpha/gamma heterodimer in nerve cells. Multifunctional enzyme that, as well as its role in glycolysis, plays a part in various processes such as growth control, hypoxia tolerance and allergic responses and may also function in the intravascular and pericellular fibrinolytic system due to its ability to serve as a receptor and activator of plasminogen on the cell surface of several cell-types such as leukocytes and neurons. ENO1 also stimulates immunoglobulin production.
Research Area: N/A
