Recombinant Human ECH1 Protein | PKSH033275

Recombinant Human ECH1 Protein | PKSH033275 | Gentaur US, UK & Europe Disrtribition

Synonyms: Delta(3, 5)-Delta(2, 4)-dienoyl-CoA isomerase; mitochondrial;ECH1;

Active Protein: N/A

Activity: Recombinant Human Delta(3, 5)-Delta(2, 4)-dienoyl-CoA isomerase, mitochondrial is produced by our E.coli expression system and the target gene encoding Thr34-Leu328 is expressed with a 6His tag at the N-terminus.

Protein Construction: Recombinant Human Delta(3, 5)-Delta(2, 4)-dienoyl-CoA isomerase, mitochondrial is produced by our E.coli expression system and the target gene encoding Thr34-Leu328 is expressed with a 6His tag at the N-terminus.

Fusion Tag: N-6His

Species: Human

Expressed Host: E.coli

Shipping: This product is provided as liquid. It is shipped at frozen temperature with blue ice/gel packs. Upon receipt, store it immediately at<-20°C.

Purity: > 95 % as determined by reducing SDS-PAGE.

Endotoxin: < 1.0 EU per µg as determined by the LAL method.

Stability and Storage: Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles.

Molecular Mass: 34.5 kDa

Formulation: Supplied as a 0.2 μm filtered solution of 20mM Tris, 100mM NaCl, 10% Glycerol, pH 8.0.

Reconstitution: Not Applicable

Background: Human delta(3, 5)-Delta(2, 4)-dienoyl-CoA isomerase(ECH1) is a member of the hydratase/isomerase superfamily and contains a C-terminal peroxisomal targeting sequence and localizes to peroxisomes. ECH1 shows high sequence similarity to enoyl-CoA hydratases of several species, particularly within a conserved domain characteristic of these proteins. The rat orthologlocalizes to the matrix of both the peroxisome and mitochondria.It can isomerize 3-trans, 5-cis-dienoyl-CoA to 2-trans, 4-trans-dienoyl-CoA, indicating that it is a delta3, 5-delta2, 4-dienoyl-CoA isomerase. ECH1 plays an important role in the auxiliary step of the fatty acid beta-oxidation pathway.

Research Area: Signal Transduction, Tags & Cell Markers, Cardiovascular, Cancer, metabolism,