Recombinant Human DFFA Protein (His Tag) | PKSH032359

Recombinant Human DFFA Protein (His Tag) | PKSH032359 | Gentaur US, UK & Europe Disrtribition

Synonyms: DNA Fragmentation Factor Subunit Alpha; DNA Fragmentation Factor 45 kDa Subunit; DFF-45; Inhibitor of CAD; ICAD; DFFA; DFF1; DFF45; H13

Active Protein: N/A

Activity: Recombinant Human DNA Fragmentation Factor Subunit alpha is produced by our E.coli expression system and the target gene encoding Met1-Thr331 is expressed with a 6His tag at the N-terminus.

Protein Construction: Recombinant Human DNA Fragmentation Factor Subunit alpha is produced by our E.coli expression system and the target gene encoding Met1-Thr331 is expressed with a 6His tag at the N-terminus.

Fusion Tag: N-6His

Species: Human

Expressed Host: E.coli

Shipping: This product is provided as liquid. It is shipped at frozen temperature with blue ice/gel packs. Upon receipt, store it immediately at<-20°C.

Purity: > 95 % as determined by reducing SDS-PAGE.

Endotoxin: < 1.0 EU per µg as determined by the LAL method.

Stability and Storage: Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles.

Molecular Mass: 38.7 kDa

Formulation: Supplied as a 0.2 μm filtered solution of 20mM PB, 150mM NaCl, pH7.4.

Reconstitution: Not Applicable

Background: DNA Fragmentation Factor Subunit Alpha (DFFA). DFFA exists as a heterodimer (DFF) with DFFB. DFF is activated once DFFA is cleaved by Caspase-3. The cleaved fragments of DFFA detach from DFFB (the active component of DFF), which in turn triggers DNA fragmentation as well as chromatin condensation during apoptosis. A reduced level of DFFA detected in ovarian endometriosis may be a part of an apoptosis-resistant mechanism enhancing the disease progression.

Research Area: Cell biology, Cancer, epigenetics and nuclear signal, metabolism