Recombinant Human DDR1 Kinase/MCK10 Protein (aa 444-913, His & GST Tag)(Active) | PKSH030389

Recombinant Human DDR1 Kinase/MCK10 Protein (aa 444-913, His & GST Tag)(Active) | PKSH030389 | Gentaur US, UK & Europe Disrtribition

Synonyms: CAK;CD167;DDR;EDDR1;HGK2;MCK10;NEP;NTRK4;PTK3;PTK3A;RTK6;TRKE

Active Protein: Active protein

Activity: A DNA sequence encoding the human DDR1 (Q08345-1) (Arg444-Val913) was fused with the N-terminal polyhistidine-tagged GST tag at the N-terminus.

Protein Construction: A DNA sequence encoding the human DDR1 (Q08345-1) (Arg444-Val913) was fused with the N-terminal polyhistidine-tagged GST tag at the N-terminus.

Fusion Tag: N-His & GST

Species: Human

Expressed Host: Baculovirus-Insect Cells

Shipping: This product is provided as liquid. It is shipped at frozen temperature with blue ice/gel packs. Upon receipt, store it immediately at<-20°C.

Purity: > 89 % as determined by reducing SDS-PAGE.

Endotoxin: < 1.0 EU per µg as determined by the LAL method.

Stability and Storage: Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles.

Molecular Mass: 80 kDa

Formulation: Supplied as sterile 20mM Tris, 500mM NaCl, pH 7.4, 10% glycerol, 3mM DTT

Reconstitution: Not Applicable

Background: Discoidin domain receptor family, member 1 (DDR1), also known as or CD167a (cluster of differentiation 167a), and Mammary carcinoma kinase 10 (MCK10), belongs to a subfamily of tyrosine kinase receptors with an extracellular domain homologous to Dictyostellium discoideum protein discoidin 1. Receptor tyrosine kinases play a key role in the communication of cells with their microenvironment. These kinases are involved in the regulation of cell growth, differentiation and metabolism. Expression of DDR1/MCK10/CD167 is restricted to epithelial cells, particularly in the kidney, lung, gastrointestinal tract, and brain. In addition, it has been shown to be significantly overexpressed in several human tumors. DDR1/MCK10/CD167 plays an important role in regulating attachment to collagen, chemotaxis, proliferation, and MMP production in smooth muscle cells. DDR1 functions in a feedforward loop to increase p53 levels and at least some of its effectors. Inhibition of DDR1 function resulted in strikingly increased apoptosis of wild-type p53-containing cells in response to genotoxic stress through a caspase-dependent pathway.

Research Area: N/A