Description
Recombinant Human DAPK3/ZIPK Protein (GST Tag)(Active) | PKSH030384 | Gentaur US, UK & Europe Disrtribition
Synonyms: DLK;ZIP;ZIPK
Active Protein: Active protein
Activity: A DNA sequence encoding the full length of human DAPK3 (NP_001339.1) (Met 1-Arg 454) was fused with the GST tag at the N-terminus.
Protein Construction: A DNA sequence encoding the full length of human DAPK3 (NP_001339.1) (Met 1-Arg 454) was fused with the GST tag at the N-terminus.
Fusion Tag: N-GST
Species: Human
Expressed Host: Baculovirus-Insect Cells
Shipping: This product is provided as liquid. It is shipped at frozen temperature with blue ice/gel packs. Upon receipt, store it immediately at<-20°C.
Purity: > 85 % as determined by reducing SDS-PAGE.
Endotoxin: < 1.0 EU per µg as determined by the LAL method.
Stability and Storage: Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles.
Molecular Mass: 79 kDa
Formulation: Supplied as sterile 20mM Tris, 500mM NaCl, 10mM GSH, pH 7.4
Reconstitution: Not Applicable
Background: Death-associated protein kinase 3, also known as DAP kinase 3, ZIP-kinase, DAPK3 and ZIPK, is a nucleus and cytoplasm protein which belongs to the protein kinase superfamily, CAMK Ser/Thr protein kinase family and DAP kinase subfamily. DAPK3 / ZIPK contains one protein kinase domain. It is a serine/threonine kinase which acts as a positive regulator of apoptosis. It phosphorylates histone H3 on 'Thr-11' at centromeres during mitosis. DAPK3 / ZIPK is a homodimer or forms heterodimers with ATF4. Both interactions require an intact leucine zipper domain and oligomerization is required for full enzymatic activity. It also binds to DAXX and PAWR, possibly in a ternary complex which plays a role in caspase activation. DAPK3 / ZIPK regulates myosin light chain phosphatase through phosphorylation of MYPT1 thereby regulating the assembly of the actin cytoskeleton, cell migration, invasiveness of tumor cells, smooth muscle contraction and neurite outgrowth. It is involved in the formation of promyelocytic leukemia protein nuclear body (PML-NB), one of many subnuclear domains in the eukaryotic cell nucleus, and which is involved in oncogenesis and viral infection.
Research Area: Signal Transduction, Cell biology, Cancer,
