Recombinant Human CSNK1G2 Protein (His Tag)(Active) | PKSH030337

Recombinant Human CSNK1G2 Protein (His Tag)(Active) | PKSH030337 | Gentaur US, UK & Europe Disrtribition

Synonyms: Casein kinase I isoform gamma-2; CK1G2; CSNK1G2

Active Protein: Active protein

Activity: A DNA sequence encoding the mature form of human CSNK1G2 (P78368-1) (Met 18-Lys 415) was expressed; with a polyhistidine tag at the N-terminus.

Protein Construction: A DNA sequence encoding the mature form of human CSNK1G2 (P78368-1) (Met 18-Lys 415) was expressed; with a polyhistidine tag at the N-terminus.

Fusion Tag: N-His

Species: Human

Expressed Host: Baculovirus-Insect Cells

Shipping: This product is provided as liquid. It is shipped at frozen temperature with blue ice/gel packs. Upon receipt, store it immediately at<-20°C.

Purity: > 80 % as determined by reducing SDS-PAGE.

Endotoxin: < 1.0 EU per µg of the protein as determined by the LAL method.

Stability and Storage: Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles.

Molecular Mass: 47.8 kDa

Formulation: Supplied as sterile 20mM Tris, 500mM NaCl, 10% gly, 1mM DTT, pH 8.0

Reconstitution: Not Applicable

Background: Casein kinase I gamma 2 isoform (CSNK1G2); a member of the large casein kinase I (CKI) subfamily; protein kinase superfamily. It may affect the development of brain; and associate with vesicular trafficking and neurotransmitter releasing from small synaptic vesicles. The CKI family includes several other isoforms (alpha; beta; gamma; and delta). Dishevelled (Dsh); another positive component of the Wnt pathway; becomes phosphorylated in response to Wnt signals. All the CKI isoforms; with the exception of gamma; increase the phosphorylation of Dsh in vivo. Casein kinase 1 gamma (CK1gamma; or CSNK1G) is associated with the cell membrane and binds to LRP. CK1gamma was found to be needed for Wnt signaling through Wnt receptor LRP. CSNK1G2 inhibits Smad3-mediated TGF-beta responses including induction of target genes and cell growth arrest; and this inhibition is dependent on CSNK1G2 kinase activity. The overexpression of CSNK1G2 in human cancers; may act as an oncoprotein during tumorigenesis. In addition; as an MTA1s-binding protein; CSNK1G2 could further potentiate the estrogen receptor (ER) corepressive function of MTA1s.

Research Area: N/A