Recombinant Human CDK7 & CCNH & MNAT1 Heterotrimer Protein (His Tag)(Active) | PKSH030310

Recombinant Human CDK7 & CCNH & MNAT1 Heterotrimer Protein (His Tag)(Active) | PKSH030310 | Gentaur US, UK & Europe Disrtribition

Synonyms: CAK1;CDKN7;HCAK;MO15;p39MO15;STK1

Active Protein: Active protein

Activity: A DNA sequence encoding the human CDK7 (P50613) (Ala 2-Phe 346) was fused with a polyhistidine tag at the N-terminus, constructed the plasmid 1; A DNA sequence encoding the human CCNH (P51946) (Tyr 2-Leu 323) was fused with a polyhistidine tag at the N-terminus, constructed the plasmid 2. A DNA sequence encoding the human MNAT1 (P51948) (Asp 2-Ser 309) was fused with a polyhistidine tag at the N-terminus, constructed the plasmid 3. The three plasmids were co-expressed and the heterotrimer was purified.

Protein Construction: A DNA sequence encoding the human CDK7 (P50613) (Ala 2-Phe 346) was fused with a polyhistidine tag at the N-terminus, constructed the plasmid 1; A DNA sequence encoding the human CCNH (P51946) (Tyr 2-Leu 323) was fused with a polyhistidine tag at the N-terminus, constructed the plasmid 2. A DNA sequence encoding the human MNAT1 (P51948) (Asp 2-Ser 309) was fused with a polyhistidine tag at the N-terminus, constructed the plasmid 3. The three plasmids were co-expressed and the heterotrimer was purified.

Fusion Tag: N-His

Species: Human

Expressed Host: Baculovirus-Insect Cells

Shipping: This product is provided as liquid. It is shipped at frozen temperature with blue ice/gel packs. Upon receipt, store it immediately at<-20°C.

Purity: > 85 % as determined by reducing SDS-PAGE.

Endotoxin: < 1.0 EU per µg of the protein as determined by the LAL method.

Stability and Storage: Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles.

Molecular Mass: 118.8 (41.2 + 39.7 + 37.9) kDa

Formulation: Supplied as sterile PBS, pH 7.4

Reconstitution: Not Applicable

Background: N/A

Research Area: Cell biology, epigenetics and nuclear signal