Recombinant Human CDK2 Protein (E.coli, His Tag) | PKSH033407

Recombinant Human CDK2 Protein (E.coli, His Tag) | PKSH033407 | Gentaur US, UK & Europe Disrtribition

Synonyms: Cyclin-Dependent Kinase 2; Cell Division Protein Kinase 2; p33 Protein Kinase; CDK2; CDKN2

Active Protein: N/A

Activity: Recombinant Human Cyclin-Dependent Kinase 2 is produced by our E.coli expression system and the target gene encoding Met1-Leu298 is expressed with a 6His tag at the N-terminus.

Protein Construction: Recombinant Human Cyclin-Dependent Kinase 2 is produced by our E.coli expression system and the target gene encoding Met1-Leu298 is expressed with a 6His tag at the N-terminus.

Fusion Tag: N-6His

Species: Human

Expressed Host: E.coli

Shipping: This product is provided as liquid. It is shipped at frozen temperature with blue ice/gel packs. Upon receipt, store it immediately at<-20°C.

Purity: > 90 % as determined by reducing SDS-PAGE.

Endotoxin: < 1.0 EU per µg as determined by the LAL method.

Stability and Storage: Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles.

Molecular Mass: 36.1 kDa

Formulation: Supplied as a 0.2 μm filtered solution of 20mM TrisHCl, 200mM NaCl, 1mM DTT, 40% Glycerol, pH 8.0.

Reconstitution: Not Applicable

Background: Cyclin-dependent kinase 2 (CDK2) belongs to the cyclin-dependent kinase of Ser/Thr protein kinase. CDK2 acts as a catalytic subunit of the cyclin dependent kinase complex, whose activity is restricted to the G1-S phage of the cell cycle, it is essential for the G1/S transition. The kinase activity of CDK2 can be regulated by the association with a cyclin subunit, its phosphorylation state and CDK inhibitors. The activation of the CDK2/cyclin complex requires the phosphorylation of Thr160 and the dephosphorylation of Try14 and Tyr15. The inhibition of CDK2-cyclin complex can also be attributed to association with p27Kip1 and p21Waf1/Cip1. The activation of CDK2 has been shown to be necessary for apoptosis of quiescent cells, such as neurons, thymocytes and endothelial cells.

Research Area: Signal Transduction, Cell biology, Neuroscience, Cancer, epigenetics and nuclear signal, metabolism,