Recombinant Human CDCP1/CD318 Protein (aa 30-341, His Tag) | PKSH033302

Recombinant Human CDCP1/CD318 Protein (aa 30-341, His Tag) | PKSH033302 | Gentaur US, UK & Europe Disrtribition

Synonyms: CUB domain-containing protein 1;Membrane glycoprotein gp140;Subtractive immunization M plus HEp3-associated 135 kDa protein;SIMA135;Transmembrane and associated with src kinases;CD318;TRASK

Active Protein: N/A

Activity: Recombinant Human CUB domain-containing protein 1 is produced by our Mammalian expression system and the target gene encoding Phe30-Ser341 is expressed with a 6His tag at the C-terminus.

Protein Construction: Recombinant Human CUB domain-containing protein 1 is produced by our Mammalian expression system and the target gene encoding Phe30-Ser341 is expressed with a 6His tag at the C-terminus.

Fusion Tag: C-6His

Species: Human

Expressed Host: Human Cells

Shipping: This product is provided as lyophilized powder which is shipped with ice packs.

Purity: > 90 % as determined by reducing SDS-PAGE.

Endotoxin: < 1.0 EU per µg as determined by the LAL method.

Stability and Storage: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.

Molecular Mass: 36.1 kDa

Formulation: Lyophilized from a 0.2 μm filtered solution of 20mM PB, 150mM NaCl, pH7.4.

Reconstitution: Please refer to the printed manual for detailed information.

Background: CUB domain-containing protein 1(CDCP1) is a transmembrane glycoprotein with a large extracellular domain (ECD) containing two CUB domains; and a smaller intracellular domain (ICD) containing five tyrosines. CDCP1 is widely expressed in human epithelial tissues; but its phosphorylation is only seen in mitotically detached or shedding cells; consistent with its role in the negative regulation of cell adhesion. The tyrosine phosphorylation of CDCP1 in cultured cells occurs when cells are induced to detach by trypsinor EDTA; or seen spontaneously during mitotic detachment. The overexpression of CDCP1 leads to the loss of cell adhesion and a detached phenotype.

Research Area: N/A