Description
Recombinant Human CDCP1/CD318 Protein (aa 1-343, His Tag) | PKSH030670 | Gentaur US, UK & Europe Disrtribition
Synonyms: CUB domain-containing protein 1;Membrane glycoprotein gp140;Subtractive immunization M plus HEp3-associated 135 kDa protein;SIMA135;Transmembrane and associated with src kinases;CD318;TRASK
Active Protein: N/A
Activity: A DNA sequence encoding the human CDCP1 (Q9H5V8-3) (Met1-Glu343) was expressed with a polyhistidine tag at the C-terminus.
Protein Construction: A DNA sequence encoding the human CDCP1 (Q9H5V8-3) (Met1-Glu343) was expressed with a polyhistidine tag at the C-terminus.
Fusion Tag: C-His
Species: Human
Expressed Host: HEK293 Cells
Shipping: This product is provided as lyophilized powder which is shipped with ice packs.
Purity: > 90 % as determined by reducing SDS-PAGE.
Endotoxin: < 1.0 EU per µg of the protein as determined by the LAL method.
Stability and Storage: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.
Molecular Mass: 36.4 kDa
Formulation: Lyophilized from sterile PBS, pH 7.4
Reconstitution: Please refer to the printed manual for detailed information.
Background: CDCP1 contains three extracellular CUB domains. It is a putative stem cell marker that is highly expressed in some human cancer cells and in both; typical and atypical (cancerous) colons. It interacts with CDH2/N-cadherin; CDH3/P-cadherin; SDC1/syndecan-1; SDC4/syndecan-4 and the serine protease ST14/MT-SP1. It also interacts with SRC and PRKCG/protein kinase C gamma. CDCP1 is taken as a key regulator of EGF/EGFR-induced cell migration. It has been shown that signaling via EGF/EGFR induces migration of ovarian cancer Caov3 and OVCA420 cells with concomitant up-regulation of CDCP1 mRNA and protein. Consistent with a role in cell migration CDCP1 relocates from cell-cell junctions to punctate structures on filopodia after activation of EGFR. It may be involved in cell adhesion and cell matrix association. It also may play a role in the regulation of anchorage versus migration or proliferation versus differentiation via its phosphorylation. It has been taken as a novel marker for leukemia diagnosis and for immature hematopoietic stem cell subsets.
Research Area: N/A
