Description
Recombinant Human CD73/NT5E Protein (His Tag)(Active) | PKSH032030 | Gentaur US, UK & Europe Disrtribition
Synonyms: 5'-Nucleotidase; 5'-NT; Ecto-5'-Nucleotidase; CD73; NT5E; NT5; NTE;CALJA;CD73;E5NT;eN;eNT;NT
Active Protein: Active protein
Activity: Recombinant Human 5'-Nucleotidase is produced by our Mammalian expression system and the target gene encoding Trp27-Lys547 is expressed with a 6His tag at the C-terminus.
Protein Construction: Recombinant Human 5'-Nucleotidase is produced by our Mammalian expression system and the target gene encoding Trp27-Lys547 is expressed with a 6His tag at the C-terminus.
Fusion Tag: C-6His
Species: Human
Expressed Host: Human Cells
Shipping: This product is provided as liquid. It is shipped at frozen temperature with blue ice/gel packs. Upon receipt, store it immediately at<-20°C.
Purity: > 90 % as determined by reducing SDS-PAGE.
Endotoxin: < 1.0 EU per µg as determined by the LAL method.
Stability and Storage: Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles.
Molecular Mass: 58.8 kDa
Formulation: Supplied as a 0.2 μm filtered solution of 20mM TrisHCl, 120mM NaCl, 4mM CaCl2, 20% Glycerol, pH 7.5.
Reconstitution: Not Applicable
Background: CD73 is a glycosyl phosphatidylinositol (GPI) anchored membrane protein that belongs to the 5'-nucleotidase family. CD73 is an ecto 5'Nucleotidase expressed by most cell types. CD73 hydrolyzes extracellular nucleotides into membrane permeable nucleosides. CD73 is one of several enzymes responsible for the production of extracellular adenosine, a signaling molecule that is involved in responses to inflammation and tissue injury. CD73 is a lymphocyte maturation marker that has functions independent of its catalytic activity. CD73 is also a regulator of leukocyte extravasation, a function that requires its 5'Nucleotidase activity. Defects in NT5E are the cause of calcification of joints and arteries (CAJA). The recombinant CD73 lacking GPI anchor is secreted as a monomer.
Research Area: Cardiovascular
