Recombinant Human CD50/ICAM-3 Protein (His Tag)(Active) | PKSH031675

Recombinant Human CD50/ICAM-3 Protein (His Tag)(Active) | PKSH031675 | Gentaur US, UK & Europe Disrtribition

Synonyms: CD50;CDW50;ICAM-3;ICAM-R

Active Protein: Active protein

Activity: A DNA sequence encoding the extracellular domain of human ICAM3 (NP_002153.2) (Met 1-His 485) was expressed, with the C-terminal fused polyhistidine tag.

Protein Construction: A DNA sequence encoding the extracellular domain of human ICAM3 (NP_002153.2) (Met 1-His 485) was expressed, with the C-terminal fused polyhistidine tag.

Fusion Tag: C-His

Species: Human

Expressed Host: HEK293 Cells

Shipping: This product is provided as lyophilized powder which is shipped with ice packs.

Purity: > 92 % as determined by reducing SDS-PAGE.

Endotoxin: < 1.0 EU per µg as determined by the LAL method.

Stability and Storage: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.

Molecular Mass: 50.8 kDa

Formulation: Lyophilized from sterile PBS, pH 7.4，5% Trehalose, 5% Mannitol, 0.01% Tween-80.

Reconstitution: Please refer to the printed manual for detailed information.

Background: The protein ICAM-3, also known as CD50, is a member of the intercellular adhesion molecule (ICAM) family consisting three members. It is a DC-SIGN ligand that is constitutively expressed on resting leukocytes, and is thus an important molecule for the first immune response. ICAM-3 comprises of five immunoglobulin-like domains, and binds LFA-1 through its two N-terminal domains. It functions not only as an adhesion molecule, but also as a potent signalling molecule. ICAM-3 binds to LFA-1 on antigen-presenting cells (APC) stabilizing the T cell-APC interaction, facilitating signaling through the CD3/TCR complex. However, recent evidence using cultured and transformed T cells suggests ICAM-3 may also function in signaling. It has been reported that CD50 molecule can play a role in developing functionally mature T lymphocytes and its expression increases during the maturation process of T lymphocytes. In addition, the interactions of ICAM-3 and LFA-1 facilitate HIV-1- induced virological synapse formation between T cells. ICAM-3 is associated with an increase of cellular radio-resistance and cancer cell proliferation. It could be considered as a candidate for anti-cancer drug development and as a cancer diagnostic marker.

Research Area: Signal Transduction, immunology,