Description
Recombinant Human CD4/LEU3 Protein (His Tag) | PKSH033506 | Gentaur US, UK & Europe Disrtribition
Synonyms: T-cell surface glycoprotein CD4;T-cell surface antigen T4/Leu-3;CD4;Scd4;CD4mut
Active Protein: N/A
Activity: Recombinant Human CD4 is produced by our Mammalian expression system and the target gene encoding Lys26-Trp390 is expressed with a 6His tag at the C-terminus.
Protein Construction: Recombinant Human CD4 is produced by our Mammalian expression system and the target gene encoding Lys26-Trp390 is expressed with a 6His tag at the C-terminus.
Fusion Tag: C-His
Species: Human
Expressed Host: Human Cells
Shipping: This product is provided as lyophilized powder which is shipped with ice packs.
Purity: > 95 % as determined by reducing SDS-PAGE.
Endotoxin: < 1.0 EU per μg as determined by the LAL method.
Stability and Storage: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.
Molecular Mass: 41.7 kDa
Formulation: Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.
Reconstitution: Please refer to the printed manual for detailed information.
Background: CD4 is an approximately 55 kDa type I transmembrane glycoprotein that is expressed predominantly on thymocytes and a subset of mature T lymphocytes. It is a standard phenotype marker for the identification of T cell populations. Mature human CD4 consists of a 371 amino acid extracellular region containing four immunoglobulin-like domains; a 22 aa transmembrane segment; and a 40 aa cytoplasmic domain. CD4 is expressed along with CD8 on double positive T cells during their development in the thymus. CD4 binds directly to MHC class II molecules on antigen presenting cells (10). This interaction contributes to the formation of the immunological synapse which is focused around the TCR-MHC class II-antigenic peptide interaction. CD4 also functions as a chemotactic receptor for IL-16 and; in human; as a co-receptor for the gp120 surface glycoprotein of HIV-1.
Research Area: immunology, Stem cells
