Recombinant Human Cathepsin D/CTSD Protein (His Tag)(Active) | PKSH030822

Recombinant Human Cathepsin D/CTSD Protein (His Tag)(Active) | PKSH030822 | Gentaur US, UK & Europe Disrtribition

Synonyms: Cathepsin D; CTSD;CLN10;CPSD;HEL-S-130P

Active Protein: Active protein

Activity: A DNA sequence encoding the pro form of human CTSD (P07339) (Met 1-Leu 412) was fused with a polyhistidine tag at the C-terminus.

Protein Construction: A DNA sequence encoding the pro form of human CTSD (P07339) (Met 1-Leu 412) was fused with a polyhistidine tag at the C-terminus.

Fusion Tag: C-His

Species: Human

Expressed Host: HEK293 Cells

Shipping: This product is provided as lyophilized powder which is shipped with ice packs.

Purity: > 97 % as determined by reducing SDS-PAGE.

Endotoxin: < 1.0 EU per µg as determined by the LAL method.

Stability and Storage: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.

Molecular Mass: 44 kDa

Formulation: Lyophilized from sterile 25mM MES, 150mM NaCl, pH 6.5

Reconstitution: Please refer to the printed manual for detailed information.

Background: Cathepsin D (CTSD); a well known lysosomal aspartyl protease and belongs to the peptidase C1 family; which is a normal and major component of lysosomes; and is found in almost all cells and tissues of mammals. Its mostly described function is intracellular catabolism in lysosomal compartments; other physiological effect include hormone and antigen processing. Cathepsin D has a specificity similar to but narrower than that of pepsin A. Cathepsin D plays an important role in the degradation of proteins; the generation of bioactive proteins; antigen processing; etc. Among different role in cell physiology; a new function of this enzyme is examined. Cathepsin D is an important regulator of apoptotic pathways in cells. It acts at different stage of intrinsic and extrinsic pathway of apoptosis. In addition; CTSD secreted from human prostate carcinoma cells are responsible for the generation of angiostatin; a potent endogenous inhibitor of angiogenesis; suggesting its contribution to the prevention of tumor growth and angiogenesis-dependent growth of metastases.

Research Area: Signal Transduction, Cell biology, Neuroscience, Cancer,