Description
Recombinant Human Cathepsin A/CTSA Protein (His Tag) | PKSH031576 | Gentaur US, UK & Europe Disrtribition
Synonyms: Lysosomal protective protein;CTSA;Carboxypeptidase C;Carboxypeptidase L;Cathepsin A;GLB2;GSL;NGBE;PPCA;PPGB
Active Protein: N/A
Activity: A DNA sequence encoding the human cathepsin A isoform b (Met 1-Tyr 480) (NP_001121167.1) was expressed with a N-terminal signal peptide and a C-terminal polyhistidine tag.
Protein Construction: A DNA sequence encoding the human cathepsin A isoform b (Met 1-Tyr 480) (NP_001121167.1) was expressed with a N-terminal signal peptide and a C-terminal polyhistidine tag.
Fusion Tag: C-His
Species: Human
Expressed Host: HEK293 Cells
Shipping: This product is provided as lyophilized powder which is shipped with ice packs.
Purity: > 90 % as determined by reducing SDS-PAGE.
Endotoxin: < 1.0 EU per µg as determined by the LAL method.
Stability and Storage: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.
Molecular Mass: 53 kDa
Formulation: Lyophilized from sterile 25mM Tris, 0.15mM NaCl, pH 7.5
Reconstitution: Please refer to the printed manual for detailed information.
Background: Lysosomal carboxypeptidase, cathepsin A (protective protein, CathA), is a component of the lysosomal multienzyme complex along with beta-galactosidase (GAL) and sialidase Neu1, where it activates Neu1 and protects GAL and Neu1 against the rapid proteolytic degradation. Cathepsin A is a multicatalytic enzyme with deamidase and esterase in addition to carboxypeptidase activities. It was recently identified in human platelets as deamidase. In vitro, it hydrolyzes a variety of bioactive peptide hormones including tachykinins, suggesting that extralysosomal cathepsin A plays a role in regulation of bioactive peptide functions. It is a member of the alpha/beta hydrolase fold family and has been suggested to share a common ancestral relationship with other alpha/beta hydrolase fold enzymes, such as cholinesterases. Cathepsin A defects are linked to multiple forms of Galactosialidosis with a combined secondary deficiency of beta-galactosidase and neuraminidase. Cathepsin A is a key molecule in the onset of galactosialidosis and also highlight the therapeutic acts in vivo as an endothelin-1-inactivating enzyme and strongly confirm a crucial role of this enzyme in effective elastic fiber formation.
Research Area: Signal Transduction, metabolism,
