Description
Recombinant Human Catalase/CAT Protein (His Tag) | PKSH030909 | Gentaur US, UK & Europe Disrtribition
Synonyms: MGC138422;MGC138424
Active Protein: N/A
Activity: A DNA sequence encoding the human CAT (P04040) (Ala 2-Leu 527) was expressed; with a polyhistidine tag at the N-terminus.
Protein Construction: A DNA sequence encoding the human CAT (P04040) (Ala 2-Leu 527) was expressed; with a polyhistidine tag at the N-terminus.
Fusion Tag: N-His
Species: Human
Expressed Host: Baculovirus-Insect Cells
Shipping: This product is provided as lyophilized powder which is shipped with ice packs.
Purity: > 80 % as determined by reducing SDS-PAGE.
Endotoxin: < 1.0 EU per µg as determined by the LAL method.
Stability and Storage: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.
Molecular Mass: 61.9 kDa
Formulation: Lyophilized from sterile 50mM Tris, 100mM NaCl, pH 8.0, 10% gly
Reconstitution: Please refer to the printed manual for detailed information.
Background: Catalase is a ubiquitously expressed enzyme that catalyzes the decomposition of hydrogen peroxide to water and oxygen. It is a tetramer of four polypeptides chains containing four porphyrin heme groups that allow the enzyme to react with the hydrogen peroxide. The optimum PH of human catalase is approximately 7 and the optimum temperature is at 37 degree. Both the PH optimum and temperature for other catalases varies depending on the species. Catalase can be inhibited by a flux of O2- generated in situ by the aerobic xanthine oxidase reaction. This inhibition of catalase by O2- provides the basis for a synergism between superoxide dismutase and catalase.Such synergisms have been observed in vitro and may be significant in vivo. Catalase is used in the food industry for removing hydrogen peroxide from milk prior to cheese production. Another use is in food wrappers where it prevents food from oxidizing. Catalase is also used in the textile industry; removing hydrogen peroxide from fabrics to make sure the material is peroxide-free.
Research Area: Signal Transduction, Tags & Cell Markers, Cardiovascular, Cancer, metabolism,
