Recombinant Human Caspase-10/CASP10 Protein (His Tag) | PKSH032176

Recombinant Human Caspase-10/CASP10 Protein (His Tag) | PKSH032176 | Gentaur US, UK & Europe Disrtribition

Synonyms: Caspase-10; CASP-10; Apoptotic Protease Mch-4; FAS-Associated Death Domain Protein Interleukin-1B-Converting Enzyme 2; FLICE2; ICE-Like Apoptotic Protease 4; CASP10; MCH4

Active Protein: N/A

Activity: Recombinant Human Caspase-10 is produced by our E.coli expression system and the target gene encoding Val220-Ile480 is expressed with a 6His tag at the C-terminus.

Protein Construction: Recombinant Human Caspase-10 is produced by our E.coli expression system and the target gene encoding Val220-Ile480 is expressed with a 6His tag at the C-terminus.

Fusion Tag: C-6His

Species: Human

Expressed Host: E.coli

Shipping: This product is provided as liquid. It is shipped at frozen temperature with blue ice/gel packs. Upon receipt, store it immediately at<-20°C.

Purity: > 90 % as determined by reducing SDS-PAGE.

Endotoxin: < 1.0 EU per µg as determined by the LAL method.

Stability and Storage: Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles.

Molecular Mass: 30.1 kDa

Formulation: Supplied as a 0.2 μm filtered solution of 25mM HEPES, 10mM DTT, pH 7.5.

Reconstitution: Not Applicable

Background: Caspase-10 (CASP10) is a 521 amino acid protein member of the Cysteine-Aspartic Acid Protease (Caspase) family. CASP10 contains two DED (Death Effector) domains and is detectable in most tissues. CASP10 cleavage by Granzyme B and autocatalytic activity generate the two active subunits: Caspase-10 subunit p23/17, Caspase-10 subunit p12. Caspases are a family of cytosolic aspartate-specific cysteine proteases involved in the execution-phase of cell apoptosis, the initiation and execution. Human caspases can be subdivided into three functional groups: cytokine activation (caspase-1, -4, -5, and -13), apoptosis initiation (caspase-2, -8, -9, -and -10), and apoptosis execution (caspase-3, -6, and -7). CASP10 cleaves and activates caspases 3 and 7, but itself is processed by caspase 8. Defects in CASP10 are associated with apoptosis defects seen in type II autoimmune lymphoproliferative syndrome.

Research Area: N/A