Description
Recombinant Human Carboxypeptidase A2/CPA2 Protein (His Tag)(Active) | PKSH031569 | Gentaur US, UK & Europe Disrtribition
Synonyms: Carboxypeptidase A2; CPA2
Active Protein: Active protein
Activity: A DNA sequence encoding the pre pro form of human CPA2 (NP_001860.2) (Met 1-Tyr 417) was expressed with a C-terminal polyhistidine tag.
Protein Construction: A DNA sequence encoding the pre pro form of human CPA2 (NP_001860.2) (Met 1-Tyr 417) was expressed with a C-terminal polyhistidine tag.
Fusion Tag: C-His
Species: Human
Expressed Host: HEK293 Cells
Shipping: This product is provided as lyophilized powder which is shipped with ice packs.
Purity: > 90 % as determined by reducing SDS-PAGE.
Endotoxin: < 1.0 EU per µg as determined by the LAL method.
Stability and Storage: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.
Molecular Mass: 46 kDa
Formulation: Lyophilized from sterile 25mM Tris, 0.15mM NaCl, pH 7.4
Reconstitution: Please refer to the printed manual for detailed information.
Background: Carboxypeptidase A2 ( CPA2 ) is a secreted pancreatic procarboxy -peptidase, and cleaves the C-terminal amide or ester bond of peptides that have a free C-terminal carboxyl group. The hydrolytic action of CPA2 was identified with a preference towards long substrates with aromatic amino acids in their C-terminal end, particularly tryptophan. CPA2 comprises a signal peptide, a pro region and a mature chain, and can be activated after cleavage of the pro peptide. Three different forms of human pancreatic procarboxypeptidase A have been isolated, and the A1 and A2 forms are always secreted as monomeric proteins with different biochemical properties.
Research Area: Cell biology
