Recombinant Human Carbonic Anhydrase 5B/CA5B Protein (His Tag) | PKSH032163

Recombinant Human Carbonic Anhydrase 5B/CA5B Protein (His Tag) | PKSH032163 | Gentaur US, UK & Europe Disrtribition

Synonyms: Carbonic Anhydrase 5B Mitochondrial; Carbonate Dehydratase VB; Carbonic Anhydrase VB; CA-VB; CA5B

Active Protein: N/A

Activity: Recombinant Human Carbonic Anhydrase 5B is produced by our E.coli expression system and the target gene encoding Cys34-Pro317 is expressed with a 6His tag at the C-terminus.

Protein Construction: Recombinant Human Carbonic Anhydrase 5B is produced by our E.coli expression system and the target gene encoding Cys34-Pro317 is expressed with a 6His tag at the C-terminus.

Fusion Tag: C-6His

Species: Human

Expressed Host: E.coli

Shipping: This product is provided as liquid. It is shipped at frozen temperature with blue ice/gel packs. Upon receipt, store it immediately at<-20°C.

Purity: > 95 % as determined by reducing SDS-PAGE.

Endotoxin: < 1.0 EU per µg as determined by the LAL method.

Stability and Storage: Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles.

Molecular Mass: 33.8 kDa

Formulation: Supplied as a 0.2 μm filtered solution of 20mM TrisHCl, 100mM NaCl, pH 8.0.

Reconstitution: Not Applicable

Background: Carbonic Anhydrase 5B (CA5B) is a member of alpha-carbonic anhydrase family (CAs) that catalyze the reversible hydration of carbon dioxide. CAs is associated with many biological processes; including calcification; respiration; bone resorption; acid-base balance and the formation of aqueous humor. CA5B is highly expressed in heart; pancreas; kidney; placenta; lung; and skeletal muscle; but it is restricted to the liver. CA5B is localized in the mitochondria and shows the highest sequence similarity to the other mitochondrial CA; CA-VA. CA5B is inhibited by coumarins; sulfonamide derivatives such as acetazolamide (AZA); saccharin; and Foscarnet.

Research Area: Signal Transduction, Tags & Cell Markers, metabolism,