Description
Recombinant Human Carbonic Anhydrase 3/CA3 Protein (His Tag)(Active) | PKSH031566 | Gentaur US, UK & Europe Disrtribition
Synonyms: Carbonic Anhydrase 3; Carbonate Dehydratase III; Carbonic Anhydrase III; CA-III; CA3;Car3
Active Protein: Active protein
Activity: A DNA sequence encoding the human CA3 (NP_005172.1) (Met 1-Lys 260) was expressed, with a polyhistide tag at the C-terminus.
Protein Construction: A DNA sequence encoding the human CA3 (NP_005172.1) (Met 1-Lys 260) was expressed, with a polyhistide tag at the C-terminus.
Fusion Tag: C-His
Species: Human
Expressed Host: E.coli
Shipping: This product is provided as lyophilized powder which is shipped with ice packs.
Purity: > 95 % as determined by reducing SDS-PAGE.
Endotoxin: Please contact us for more information.
Stability and Storage: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.
Molecular Mass: 30.4 kDa
Formulation: Lyophilized from sterile 50mM Tris, 500mM NaCl, 10% glycerol, pH 8.0
Reconstitution: Please refer to the printed manual for detailed information.
Background: Carbonic anhydrases (CAs) are a large family of zinc metalloenzymes first discovered in 1933 that catalyze the reversible hydration of carbon dioxide. CAs participate in a variety of biological processes, including respiration, calcification, acid-base balance, bone resorption, and the formation of aqueous humor, cerebrospinal fluid, saliva, and gastric acid. Carbonic anhydrases (CAs) form a family of enzymes that catalyze the rapid conversion of carbon dioxide and water to bicarbonate and protons, a reaction that occurs rather slowly in the absence of a catalyst. The active site of most carbonic anhydrases contains a zinc ion, they are therefore classified as metalloenzymes. Several forms of carbonic anhydrase occur in nature. The primary function of the enzyme in animals is to interconvert carbon dioxide and bicarbonate to maintain acid-base balance in blood and other tissues, and to help transport carbon dioxide out of tissues. Plants contain a different form called β-carbonic anhydrase, which, from an evolutionary standpoint, is a distinct enzyme, but participates in the same reaction and also uses a zinc ion in its active site. Carbonic anhydrase 3, also known as Carbonate dehydratase III, CA-III and CA3, is a cytoplasm protein which belongs to the alpha-carbonic anhydrase family. CA3 is activated by proton donors such as imidazole and the dipeptide histidylhistidine. It is inhibited by coumarins and sulfonamide derivatives such as acetazolamide. At 6 weeks gestation, transcripts accumulate at low levels in the somites and at high levels throughout the notochord. As gestation continues, CA3 becomes abundant in all developing muscle masses and continues at high to moderate levels in the notochord.
Research Area: Tags & Cell Markers, Cell biology, Cardiovascular
