Description
Recombinant Human Bruton Tyrosine Kinase/BTK Kinase Protein (His Tag)(Active) | PKSH030407 | Gentaur US, UK & Europe Disrtribition
Synonyms: AGMX1;AT;ATK;BPK;IMD1;PSCTK1;XLA
Active Protein: Active protein
Activity: A DNA sequence encoding the human BTK (NP_000052.1) (Met 1-Ser 659) was expressed with a C-terminal polyhistidine tag.
Protein Construction: A DNA sequence encoding the human BTK (NP_000052.1) (Met 1-Ser 659) was expressed with a C-terminal polyhistidine tag.
Fusion Tag: C-His
Species: Human
Expressed Host: Baculovirus-Insect Cells
Shipping: This product is provided as liquid. It is shipped at frozen temperature with blue ice/gel packs. Upon receipt, store it immediately at<-20°C.
Purity: > 85 % as determined by reducing SDS-PAGE.
Endotoxin: < 1.0 EU per µg as determined by the LAL method.
Stability and Storage: Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles.
Molecular Mass: 77.8 kDa
Formulation: Supplied as sterile 20mM Tris, 500mM NaCl, 10% glycerol, pH 7.0
Reconstitution: Not Applicable
Background: Bruton's tyrosine kinase (or BTK) is a type of kinase protein expressed in B lymphocytes and T cells. BTK contains a PH domain which binds phosphatidylinositol(3, 4, 5)-trisphosphate (PIP3). After binding to PIP3, BTK is induced to phosphorylate phospholipase C, which in turn hydrolyzes PIP2 into two second messagers, IP3 and DAG, which then modulate the activity of downstream proteins during B-cell signaling. Btk is also found implicated in the primary immunodeficiency disease X-linked agammaglobulinemia(Bruton's agammaglobulinemia). BTK played a key role in B-cell maturation as well as mast cell activation through the high-affinity IgE receptor. Patients with X-linked agammaglobulinemia have normal pre-B cell populations in their bone marrow but these B-cells can not mature and enter the circulation.Immune Checkpoint Immunotherapy Cancer Immunotherapy Targeted Therapy
Research Area: Signal Transduction, immunology,
