Recombinant Human Biotinidase/BTD Protein (His Tag)(Active) | PKSH030529

Recombinant Human Biotinidase/BTD Protein (His Tag)(Active) | PKSH030529 | Gentaur US, UK & Europe Disrtribition

Synonyms: BTD

Active Protein: Active protein

Activity: A DNA sequence encoding the human BTD (P43251) (Met1-Asp543) with a C-terminal polyhistidine tag was expressed.

Protein Construction: A DNA sequence encoding the human BTD (P43251) (Met1-Asp543) with a C-terminal polyhistidine tag was expressed.

Fusion Tag: C-His

Species: Human

Expressed Host: HEK293 Cells

Shipping: This product is provided as lyophilized powder which is shipped with ice packs.

Purity: > 95 % as determined by reducing SDS-PAGE.

Endotoxin: < 1.0 EU per µg as determined by the LAL method.

Stability and Storage: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.

Molecular Mass: 58.2 kDa

Formulation: Lyophilized from sterile PBS, pH 7.4

Reconstitution: Please refer to the printed manual for detailed information.

Background: Biotinidase, also known as biotinase and BTD, is a ubiquitous mammalian cell enzyme which expressed at high levels in the liver, serum, and kidney. Its primary function is to cleave biotin from biocytin, preserving the pool of biotin for use as a cofactor for biotin dependent enzymes, namely the 4 human carboxylases. Biotinidase also recycles biotin from enzymes in the body that use it as a helper component in order to function. These enzymes, known ascarboxylases, are important in the processing of fats,  carbohydrates, and proteins. Biotin is attached to these carboxylase enzymes through an amino acid (the building material of proteins) called lysine, forming a complex calledbiocytin.

Research Area: Signal Transduction, Neuroscience, metabolism,