Description
Recombinant Human BCL2/Bcl-2 Protein (His Tag) | PKSH033761 | Gentaur US, UK & Europe Disrtribition
Synonyms: Apoptosis regulator Bcl-2; BCL2; Apoptosis Regulator Bcl-2; B-cell Lymphoma 2;PPP1R50
Active Protein: N/A
Activity: Recombinant Human BCL2 is produced by our E.coli expression system and the target gene encoding Met1-Asp211 is expressed with a 6His tag at the C-terminus.
Protein Construction: Recombinant Human BCL2 is produced by our E.coli expression system and the target gene encoding Met1-Asp211 is expressed with a 6His tag at the C-terminus.
Fusion Tag: C-His
Species: Human
Expressed Host: E.coli
Shipping: This product is provided as liquid. It is shipped at frozen temperature with blue ice/gel packs. Upon receipt, store it immediately at<-20°C.
Purity: > 95 % as determined by reducing SDS-PAGE.
Endotoxin: < 1.0 EU per μg as determined by the LAL method.
Stability and Storage: Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles.
Molecular Mass: 24.1 kDa
Formulation: Supplied as a 0.2 μm filtered solution of 20mM HEPES, 150mM NaCl, 10%Glycerol, pH8.0.
Reconstitution: Not Applicable
Background: Bcl-2 is a member of a family of proteins that regulates outer mitochondrial membrane permeability. Bcl-2 is an antiapoptotic member that prevents release of cytochrome c from the mitochondria intermembrane space into the cytosol. Bcl-2 is present on the outer mitochondrial membrane and is also found on other membranes in some cell types. BCL-2 is localized to the outer membrane of mitochondria, where it plays an important role in promoting cellular survival and inhibiting the actions of pro-apoptotic proteins. The pro-apoptotic proteins in the BCL-2 family, including Bax and Bak, normally act on the mitochondrial membrane to promote permeabilization and release of cytochrome C and ROS, that are important signals in the apotosis cascade. These pro-apoptotic proteins are in turn activated by BH3-only proteins, and are inhibited by the function of BCL-2 and its relative BCL-Xl.
Research Area: Signal Transduction, Cell biology, Cancer, metabolism,
