Recombinant Human BBOX1/Gamma-BBH Protein (His & GST Tag) | PKSH030526

Recombinant Human BBOX1/Gamma-BBH Protein (His & GST Tag) | PKSH030526 | Gentaur US, UK & Europe Disrtribition

Synonyms: BBH;BBOX;G-BBH;gamma-BBH

Active Protein: N/A

Activity: A DNA sequence encoding the human BBOX1 (O75936) (Met1-Asn387) was fused with the N-terminal polyhistidine-tagged GST tag at the N-terminus.

Protein Construction: A DNA sequence encoding the human BBOX1 (O75936) (Met1-Asn387) was fused with the N-terminal polyhistidine-tagged GST tag at the N-terminus.

Fusion Tag: N-His & GST

Species: Human

Expressed Host: Baculovirus-Insect Cells

Shipping: This product is provided as lyophilized powder which is shipped with ice packs.

Purity: > 85 % as determined by reducing SDS-PAGE.

Endotoxin: < 1.0 EU per µg as determined by the LAL method.

Stability and Storage: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.

Molecular Mass: 72.5 kDa

Formulation: Lyophilized from sterile 20mM Tris, 500mM NaCl, 10% glycerol, pH 8.0

Reconstitution: Please refer to the printed manual for detailed information.

Background: BBOX1, also known as gamma-BBH, belongs to the gamma-BBH/TMLD family. It is highly expressed in kidney and moderately expressed in liver. BBOX1 catalyzes the formation of L-carnitine from gamma-butyrobetaine, the last step in the L-carnitine biosynthetic pathway. Carnitine is essential for the transport of activated fatty acids across the mitochondrial membrane during mitochondrial beta-oxidation. BBOX1 is an inhibition target for mildronate which can be used to treat angina andmyocardial infarction. Mildronate may also be beneficial for the treatment ofneurological disorder,  diabetes, and seizures and alcohol intoxication.

Research Area: Signal Transduction, metabolism,