Recombinant Human B3GAT3 Protein (His Tag) | PKSH033257

Recombinant Human B3GAT3 Protein (His Tag) | PKSH033257 | Gentaur US, UK & Europe Disrtribition

Synonyms: B3GAT3;Galactosylgalactosylxylosylprotein 3-beta-glucuronosyltransferase 3;Beta-1;3-glucuronyltransferase 3;Glucuronosyltransferase I;GlcAT-I;GlcUAT-I;Gal beta-1;3-Gal-R glucuronyltransferase;;

Active Protein: N/A

Activity: Recombinant Human Galactosylgalactosylxylosylprotein 3-beta-glucuronosyltransferase 3 is produced by our E.coli expression system and the target gene encoding Glu72-Val335 is expressed with a 6His tag at the C-terminus.

Protein Construction: Recombinant Human Galactosylgalactosylxylosylprotein 3-beta-glucuronosyltransferase 3 is produced by our E.coli expression system and the target gene encoding Glu72-Val335 is expressed with a 6His tag at the C-terminus.

Fusion Tag: C-6His

Species: Human

Expressed Host: E.coli

Shipping: This product is provided as liquid. It is shipped at frozen temperature with blue ice/gel packs. Upon receipt, store it immediately at<-20°C.

Purity: > 95 % as determined by reducing SDS-PAGE.

Endotoxin: < 1.0 EU per µg as determined by the LAL method.

Stability and Storage: Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles.

Molecular Mass: 30.4 kDa

Formulation: Supplied as a 0.2 μm filtered solution of 20mM Tris, 150mM NaC, 2mM EDTA, 20% Glycerol, pH 8.0.

Reconstitution: Not Applicable

Background: Galactosylgalactosylxylosylprotein 3-beta-glucuronosyltransferase 3 (B3GAT3) is an enzyme that in humans is encoded by the B3GAT3 gene, belongs to the glycosyltransferase 43 family. B3GAT3 is involved in a number of biological processes such as catalyzing the formation of the glycosaminoglycan-protein linkage by way of a glucuronyl transfer reaction in the final step of the biosynthesis of the linkage region of proteoglycans, forming the linkage tetrasaccharide present in heparan sulfate and chondroitin sulfate, gGlycosaminoglycans biosynthesis, transfering a glucuronic acid moiety from the uridine diphosphate-glucuronic acid (UDP-GlcUA) to the common linkage region trisaccharide Gal-beta-1, 3-Gal-beta-1, 4-Xyl covalently bound to a Ser residue at the glycosaminylglycan attachment site of proteoglycans.It also plays a role in the biosynthesis of l2/HNK-1 carbohydrate epitope on glycoproteins, hows strict specificity for Gal-beta-1, 3-Gal-beta-1, 4-Xyl, exhibiting negligible incorporation into other galactoside substrates including Galbeta1-3Gal beta1-O-benzyl, Galbeta1-4GlcNAc and Galbeta1-4Glc and stimulates 2-phosphoxylose phosphatase activity of PXYLP1 in presence of uridine diphosphate-glucuronic acid (UDP-GlcUA) during completion of linkage region formation.

Research Area: Signal Transduction, metabolism,