Recombinant Human ATG10 Protein (His&T7 Tag) | PKSH032097

Recombinant Human ATG10 Protein (His&T7 Tag) | PKSH032097 | Gentaur US, UK & Europe Disrtribition

Synonyms: Ubiquitin-Like-Conjugating Enzyme ATG10; Autophagy-Related Protein 10; APG10-Like; ATG10

Active Protein: N/A

Activity: Recombinant Human Autophagy Related 10 Homolog is produced by our E.coli expression system and the target gene encoding Met1-Thr190 is expressed with a T7 tag at the N-terminus, 6His tag at the C-terminus.

Protein Construction: Recombinant Human Autophagy Related 10 Homolog is produced by our E.coli expression system and the target gene encoding Met1-Thr190 is expressed with a T7 tag at the N-terminus, 6His tag at the C-terminus.

Fusion Tag: C-6His

Species: Human

Expressed Host: E.coli

Shipping: This product is provided as liquid. It is shipped at frozen temperature with blue ice/gel packs. Upon receipt, store it immediately at<-20°C.

Purity: > 95 % as determined by reducing SDS-PAGE.

Endotoxin: < 1.0 EU per µg as determined by the LAL method.

Stability and Storage: Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles.

Molecular Mass: 24.5 kDa

Formulation: Supplied as a 0.2 μm filtered solution of 20mM PB, 150mM NaCl, pH 8.0.

Reconstitution: Not Applicable

Background: Ubiquitin-Like-Conjugating Enzyme ATG10 (ATG10) is a ubiquitous 28kDa member of the ATG10 family protein. ATG10 acts as an E2-like enzyme, catalyzes the transfer of ATG12 to ATG5 during in the initial stages of autophagesome formation. The heterodimer of ATG5 and ATG12 subsequntly associates non-covalently with an ATG16 multimer to generate an antophagosome. ATG10 plays a role in the conjugation of ATG12 to ATG5 by interaction with MAP1LC3A. In addition, ATG10 can diretly interact with ATG5 or ATG7.

Research Area: Cell biology, Cardiovascular, Neuroscience, Cancer, metabolism